L-Phenylalanyl-L-glutamic acid TFA (H-Phe-Glu-OH TFA) is a dipeptide present in the exudates of alfalfa seedlings, which exhibits high affinity for PEPT2. L-Phenylalanyl-L-glutamic acid TFA can serve as the peptide scaffold of a tyrosyl-tRNA synthetase inhibitor against Staphylococcus aureus [1] [2] [3].
In Vitro, L-Phenylalanyl-L-glutamic acid TFA acts as a peptide scaffold inhibitor of Staphylococcus aureus tyrosyl-tRNA synthetase[1]. L-Phenylalanyl-L-glutamic acid TFA is a high-affinity substrate of PEPT2 with a Ki of 0.022 mM; it exhibits low affinity for PEPT1, with a Ki of 0.18 mM[3].
References:
[1]. Abouzeid S, et al. Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli. Molecules. 2023 Mar 14;28(6):2645.
[2]. Farshadfar C, et al. Novel potential inhibitor discovery against tyrosyl-tRNA synthetase from Staphylococcus aureus by virtual screening, molecular dynamics, MMPBSA and QMMM simulations. Mol Simul. 2020 Mar 31;46(7):507-520.
[3]. Biegel A, et al. Structural Requirements for the Substrates of the H + /Peptide Cotransporter PEPT2 Determined by Three-Dimensional Quantitative Structure−Activity Relationship Analysis. J Med Chem. 2006;49(14):4286-4296.
In Vitro, L-Phenylalanyl-L-glutamic acid TFA acts as a peptide scaffold inhibitor of Staphylococcus aureus tyrosyl-tRNA synthetase[1]. L-Phenylalanyl-L-glutamic acid TFA is a high-affinity substrate of PEPT2 with a Ki of 0.022 mM; it exhibits low affinity for PEPT1, with a Ki of 0.18 mM[3].
References:
[1]. Abouzeid S, et al. Antibacterial and Antibiofilm Effects of Allelopathic Compounds Identified in Medicago sativa L. Seedling Exudate against Escherichia coli. Molecules. 2023 Mar 14;28(6):2645.
[2]. Farshadfar C, et al. Novel potential inhibitor discovery against tyrosyl-tRNA synthetase from Staphylococcus aureus by virtual screening, molecular dynamics, MMPBSA and QMMM simulations. Mol Simul. 2020 Mar 31;46(7):507-520.
[3]. Biegel A, et al. Structural Requirements for the Substrates of the H + /Peptide Cotransporter PEPT2 Determined by Three-Dimensional Quantitative Structure−Activity Relationship Analysis. J Med Chem. 2006;49(14):4286-4296.