LDS-751 |
Katalog-Nr.GC30134
One-Click Copy Product Info
|
LDS-751 ist ein NukleinsÄurefarbstoff, der hauptsÄchlich DNA nachweist.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 181885-68-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
LDS 751 is a cell membrane permeable nucleic acid stain used to distinguish intact nucleated cells from anucleated and damaged nucleated cells, and to distinguish different cell types in mixed neutrophils, lymphocytes and monocytes by flow cytometry. The excitation/emission light of LDS 751 is 543/712nm respectively (when bound to DNA). The maximum excitation wavelength of LDS 751 binding to dsDNA is ~543nm, which can be excited by a 488nm argon ion laser. It is particularly suitable for multicolor analysis due to its longer maximum emission wavelength (~712 nm). Binding to dsDNA causes a fluorescence enhancement of ~20 times. Thiazole orange (mainly binds RNA, GC30038) and LDS751 (mainly binds DNA) are used to distinguish red blood cells, platelets, reticulocytes and nucleated cells.
This protocol is only a guide. Please modify it according to your specific needs.
1. Prepare LDS 751 staining solution
(1) Prepare LDS 751 dye stock solution: Use 5.297mL DMSO to fully dissolve 25mg LDS 751 to obtain 10mM stock solution.
Note: It is recommended to store LDS 751 stock solution at -20℃ in the dark after aliquoting to avoid repeated freezing and thawing.
(2) Prepare LDS 751 working solution: Use preheated serum-free culture medium or buffer (such as HBSS or PBS) to dilute the stock solution and prepare LDS 751 working solution with a concentration of 10μM.
Note: Please adjust the concentration of LDS 751 working solution according to actual conditions and prepare it before use.
2. Cell staining
2.1 Suspended cells (taking 6-well plate as an example)
(1) Suspended cells are centrifuged at 1000g for 3-5min. Discard the supernatant and wash twice with PBS for 5 minutes each.
(2) Add 1 mL of LDS 751 working solution and incubate at room temperature in the dark for 15-60 min.
(3) After incubation, centrifuge at 1000g for 5 minutes, remove the supernatant, and wash 2-3 times with PBS, 5 minutes each time.
(4) Resuspend the cells in serum-free cell culture medium or PBS, and observe by fluorescence enzyme labeling plate or flow cytometry.
2.2 Adherent cells
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the culture medium, aspirate the excess culture medium, and place the coverslip in a humid environment.
(3) Add 100uL of LDS 751 working solution from one corner of the coverslip, gently shake to evenly cover all cells with the dye, and incubate at room temperature in the dark for 15-60 min.
(4) Aspirate the dye working solution, wash the coverslip 2-3 times with culture medium, and observe by fluorescence enzyme labeling plate or flow cytometry.
3. Fluorescence ELISA plate detection: The excitation/emission light of LDS 751 is 543/712nm respectively (binding to DNA)
Notes:
(1) For staining of fixed cells or tissue samples, rinse to remove the fixative after fixation;
(2) To slow down fluorescence quenching, it is recommended to use anti-fluorescence quenching sealing agent;
(3) LDS 751 is irritating to the human body. For your safety and health, please wear a lab coat and disposable gloves when operating.
(4) Be sure to try several staining concentrations for the first experiment to determine the optimal working concentration. Higher concentrations may cause nonspecific staining of cell structures.
| Cas No. | 181885-68-7 | SDF | |
| Canonical SMILES | CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O | ||
| Formula | C25H30ClN3O4 | M.Wt | 471.98 |
| Löslichkeit | DMSO : 83.33 mg/mL (176.55 mM; ultrasonic and warming and heat to 60°C) | Storage | Store at -20°C,stored under argon,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1187 mL | 10.5937 mL | 21.1873 mL |
| 5 mM | 423.7 μL | 2.1187 mL | 4.2375 mL |
| 10 mM | 211.9 μL | 1.0594 mL | 2.1187 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)