Methyl Green Staining Solution (Chloroform-Extracted, 1%)

Methyl Green Staining Solution (Chloroform-Extracted, 1%) is a basic dye stock solution with differential nucleic acid binding activity that distinguishes double-stranded DNA from RNA, and it can be used for assays related to nucleic acid distribution and DNA localization. Methyl Green Staining Solution (Chloroform-Extracted, 1%) functions by competitively binding to the phosphate groups of nucleic acids and stably binds to intact double-stranded DNA; RNA within the oocyte-specific ribonucleoprotein complex specifically binds to this dye, and RNase completely blocks this binding reaction. The nucleic acid recognition of Methyl Green Staining Solution (Chloroform-Extracted, 1%) depends on the size of the dye cation and the intact double-helical structure of DNA ^{[1] [2]}.

In Vitro, Methyl Green Staining Solution (Chloroform-Extracted, 1%) specifically labels cytoplasmic RNA and nucleoli in oocytes; the cytoplasmic staining signal is completely eliminated by RNase pretreatment, while DNase only causes weak interference^[1]. Methyl Green Staining Solution (Chloroform-Extracted, 1%) combined with Pyronin Y stains somatic cell nuclei green, while the cytoplasm and nucleoli of oocytes show Pyronin-positive staining^[1]. Methyl Green Staining Solution (Chloroform-Extracted, 1%) achieves optimal differential metachromatic staining of nucleic acids in paraffin sections fixed with paraformaldehyde-glutaraldehyde; it stains DNA blue-green and RNA reddish-purple, and shows almost no non-specific binding to pure proteins^[2].

References:
[1]. Cowden R R. The staining behavior of oocyte cytoplasm with the alcoholic Feulgen variant and with methyl green. Histochemie, 1965, 5:441‑447.
[2]. Roque A L, et al. A stain for the histochemical demonstration of nucleic acids. Experimental and Molecular Pathology, 1965, 4(3): 266‑274.