IF 13
PMID: 38899975 ↗Doxycycline
Catalog No.: GC63621Purity:>98.00%
Doxycycline is a tetracycline-derivative wide-spectrum antibiotic, inhibit protein synthesis by interfering with the binding of activated aminoacyl-tRNAs on the A-site of the 30S subunit of bacterial ribosomes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 564-25-0
25mgIn Stock
$35.00
1
50mgIn Stock
$56.00
1
100mgIn Stock
$84.00
1
500mgIn Stock
$147.00
1
1gIn Stock
$207.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 25mg | $35.00 | In Stock | 1 | |
| 50mg | $56.00 | In Stock | 1 | |
| 100mg | $84.00 | In Stock | 1 | |
| 500mg | $147.00 | In Stock | 1 | |
| 1g | $207.00 | In Stock | 1 |
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Citations
2 publicationsPublications from researchers using this productDescription
Doxycycline is a tetracycline-derivative wide-spectrum antibiotic, inhibit protein synthesis by interfering with the binding of activated aminoacyl-tRNAs on the A-site of the 30S subunit of bacterial ribosomes. It is used to treat a variety of infections caused by bacteria and protozoa. Doxycycline is also a nonspecific matrix metalloproteinase (MMP) inhibitor. Doxycycline exhibits anti-tumour and pro-apoptotic activity [1-4].
Doxycycline(0-250 µM; 72h) inhibits cell viability of breast cancer cells and mammospheres[5]. Doxycycline (100 ng/mL, 1 µg/mL; 24 h) reduces proliferation of human cell lines[6].
Doxycycline(20mg /kg; p.o.;2times) treated following mice TBI improved behavioral and motor function suggesting doxycycline's role in preserving murine BBB integrity[7]. Doxycycline(DOX in drinking water (6 mg/ml, 5 ml/day/mice) ; 36 days) significantly minimized the dystrophic phenotype of skeletal and cardiac muscles and improved forelimb muscle strength[8]. The FM(fecal microbiota) of female C57BL/6NCrl mice is significantly altered by administration of doxycycline(doxycycline in the drinking water at 2 mg/ml during weeks 1, 2, and 4) at a concentration of 2 mg/ml in the drinking water[9].
References:
[1]. Cunha BA, Sibley CM, et,al. Doxycycline. Ther Drug Monit. 1982;4(2):115-35. doi: 10.1097/00007691-198206000-00001. PMID: 7048645.
[2].Manchado E, Weissmueller S, et,al. A combinatorial strategy for treating KRAS-mutant lung cancer. Nature. 2016 Jun 30;534(7609):647-51. doi: 10.1038/nature18600. Epub 2016 Jun 22. PMID: 27338794; PMCID: PMC4939262.
[3].Luger AL, Sauer B, et,al. Doxycycline Impairs Mitochondrial Function and Protects Human Glioma Cells from Hypoxia-Induced Cell Death: Implications of Using Tet-Inducible Systems. Int J Mol Sci. 2018 May 17;19(5):1504. doi: 10.3390/ijms19051504. PMID: 29772845; PMCID: PMC5983704.
[4]. Duivenvoorden WC, Popović SV, et,al. Doxycycline decreases tumor burden in a bone metastasis model of human breast cancer. Cancer Res. 2002 Mar 15;62(6):1588-91. PMID: 11912125.
[5]. Zhang L, Xu L, et,al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745. doi: 10.1080/15384101.2016.1241929. Epub 2016 Oct 18. PMID: 27753527; PMCID: PMC5405729.
[6]. Ahler E, Sullivan WJ, et,al. Doxycycline alters metabolism and proliferation of human cell lines. PLoS One. 2013 May 31;8(5):e64561. doi: 10.1371/journal.pone.0064561. PMID: 23741339; PMCID: PMC3669316.
[7]. Malek AJ, Robinson BD, et,al. Doxycycline improves traumatic brain injury outcomes in a murine survival model. J Trauma Acute Care Surg. 2020 Sep;89(3):435-440. doi: 10.1097/TA.0000000000002801. PMID: 32467458.
[8]. Pereira JA, Taniguti AP, et,al. Doxycycline ameliorates the dystrophic phenotype of skeletal and cardiac muscles in mdx mice. Muscle Nerve. 2012 Sep;46(3):400-6. doi: 10.1002/mus.23331. PMID: 22907231.
[9]. Boynton FDD, Ericsson AC, et,al. Doxycycline induces dysbiosis in female C57BL/6NCrl mice. BMC Res Notes. 2017 Nov 29;10(1):644. doi: 10.1186/s13104-017-2960-7. PMID: 29187243; PMCID: PMC5708113.
Protocol
| Cell experiment [1]: | |
|
Cell lines |
MCF-7 and MDA-MB-468 cells |
|
Preparation Method |
Cells were seeded in 96-well plates at a density of 5000 cells/well. The cells were incubated with doxycycline at concentrations of 0-250 µM for 72 hours. |
|
Reaction Conditions |
0-250 µM; 72h |
|
Applications |
Doxycycline inhibits cell viability of breast cancer cells and mammospheres. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6J mice |
|
Preparation Method |
Mice were assigned to a sham, Traumatic brain injury (TBI), or TBI with doxycycline arm. A moderate TBI was induced utilizing a controlled cortical impactor. The TBI with doxycycline cohort received a dose of doxycycline (20 mg/kg) 2 hours after injury and every 12 hours until postoperative day (POD) 6. |
|
Dosage form |
20 mg/kg; p.o ; 2 times |
|
Applications |
Doxycycline treated following mice TBI improved behavioral and motor function. |
|
References: [1]. Zhang L, Xu L, et,al. Doxycycline inhibits the cancer stem cell phenotype and epithelial-to-mesenchymal transition in breast cancer. Cell Cycle. 2017 Apr 18;16(8):737-745. doi: 10.1080/15384101.2016.1241929. Epub 2016 Oct 18. PMID: 27753527; PMCID: PMC5405729. |
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Product Documents
Purity:>98.00%Appearance:A solid
Chemical Properties
CAS No.
564-25-0
Molecular Formula
C22H24N2O8
Molecular Weight
444.43
Solubility
DMSO : 125 mg/mL (281.26 mM; Need ultrasonic)
Storage
Store at 4°C, protect from light, stored under argon
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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