FITC-D-Glucose is a fluorescently labeled glucose derivative and an orally effective bacterial metabolic activity detection probe. FITC-D-Glucose is applicable to the research of cholera [1].
In Vivo, FITC-D-Glucose (0.1 mM; p.o.; single administration of 100 μL) labels metabolically active V. cholerae in adult mice. Studies show that the proportion of highly metabolically active cells in the ΔflhB mutant strain is 7 times that of the wild-type strain, while this proportion in the ΔflaA mutant strain is only 0.2 times that of the wild-type strain[1].
In Vitro, FITC-D-Glucose (1 μL in 10 mL Met-free MM medium; 30 min at 37 °C) labeling reveals that flhB mutant V. cholerae has 1.7-fold higher in vitro metabolic activity than wild-type, while flaA mutant has 0.6-fold lower in vitro metabolic activity than wild-type[1].
References:
[1]. Guozhong Chen, et al. Anti-σ28 factor FlgM secretion regulates Vibrio cholerae adaptability1 in adult mice through quorum sensing and methionine metabolism.
In Vivo, FITC-D-Glucose (0.1 mM; p.o.; single administration of 100 μL) labels metabolically active V. cholerae in adult mice. Studies show that the proportion of highly metabolically active cells in the ΔflhB mutant strain is 7 times that of the wild-type strain, while this proportion in the ΔflaA mutant strain is only 0.2 times that of the wild-type strain[1].
In Vitro, FITC-D-Glucose (1 μL in 10 mL Met-free MM medium; 30 min at 37 °C) labeling reveals that flhB mutant V. cholerae has 1.7-fold higher in vitro metabolic activity than wild-type, while flaA mutant has 0.6-fold lower in vitro metabolic activity than wild-type[1].
References:
[1]. Guozhong Chen, et al. Anti-σ28 factor FlgM secretion regulates Vibrio cholerae adaptability1 in adult mice through quorum sensing and methionine metabolism.