GLPBio

Fraxetin

Catalog No.: GN10556Purity:>98.00%Synonyms: 7,8-Dihydroxy-6-Methoxycoumarin
COASDSData Sheet
Fraxetin is a plant-derived O-methylcoumarin.

Products are for research use only. Not for human use. We do not sell to patients.

Fraxetin
Cas No.: 574-84-5
10mM (in 1mL DMSO)In Stock
$40.00
1
1mgIn Stock
$17.00
1
5mgIn Stock
$37.00
1
10mgIn Stock
$59.00
1
25mgIn Stock
$108.00
1
50mgIn Stock
$158.00
1
100mgIn Stock
$238.00
1
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Description

Fraxetin is a plant-derived O-methylcoumarin [1]. Fraxetin exerts anti-inflammatory effects by directly scavenging reactive oxygen free radicals and activating antioxidant defenses, and intervening in inflammatory signals [2]. Fraxetin has anti-tumor, anti-inflammatory, and neuroprotective effects [3-4].

In MCF-7 cells, Fraxetin (10-60µM; 24h, 48h) significantly inhibited cell proliferation in a concentration and time-dependent manner [5]. In SKOV3 and SW626 cells, Fraxetin (80µM; 12h) suppresses the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the TLR4/STAT3 signaling pathway [6]. In T98G and LN-229 cells, Fraxetin (5-50µM; 48h) inhibited cell viability in a concentration-dependent manner [7].

In C57BL/6J mice, Selenium-dependent glutathione reductase activity is significantly increased in the liver of Fraxetin (25mg/kg; po; 30d) treated mice [8]. In Dextran sulphate sodium (DSS)-induce a mouse model of colitis, Fraxetin (10mg/kg, 30mg/kg, 60mg/kg; po; 8d) alleviates DSS-induced colitis by modulating the inflammatory response, enhancing epithelial barrier integrity and regulating the gut microbiota [9].

References:
[1]. Wang H, Zou DA, Xie K, et al. Antibacterial mechanism of fraxetin against Staphylococcus aureus. Molecular Medicine Reports. 2014 Nov 1; 10(5): 2341-2345.
[2]. Balaha M, Ahmed N, Geddawy A, et al. Fraxetin prevented sodium fluoride-induced chronic pancreatitis in rats: Role of anti-inflammatory, antioxidant, antifibrotic and anti-apoptotic activities. International immunopharmacology. 2021 Apr 1; 93: 107372.
[3]. Ha NM, Son NT. Health benefits of fraxetin: from chemistry to medicine. Archiv der Pharmazie. 2024 Jul; 357(7): 2400092.
[4]. Fang T, Liu L, Liu W. Exploring the mechanism of fraxetin against acute myeloid leukemia through cell experiments and network pharmacology. BMC Complementary Medicine and Therapies. 2024 Jun 10; 24(1): 226.
[5]. Liu G, Liu Z, Yan Y, et al. Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters. 2017 Dec 1; 14(6): 7374-7378.
[6]. Xu R, Ruan Y, Zhang L, et al. Fraxetin suppresses the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the TLR4/STAT3 signaling pathway. Immunopharmacology and Immunotoxicology. 2023 May 4; 45(3): 287-294.
[7]. Yao H, Li X, Pan X, et al. Fraxetin exerts anticancer effect in glioma by suppressing MiR‐21‐3p. Drug Development Research. 2022 Apr; 83(2): 501-511.
[8]. MARTÍN‐ARAGÓN SA, Benedí JM, Villar AM. Modifications on antioxidant capacity and lipid peroxidation in mice under fraxetin treatment. Journal of pharmacy and pharmacology. 1997 Jan; 49(1): 49-52.
[9]. Sun X, Jin X, Wang L, et al. Fraxetin ameliorates symptoms of dextran sulphate sodium-induced colitis in mice. Heliyon. 2024 Jan 15; 10(1).

Protocol

Cell experiment [1]:

Cell lines

MCF-7 cells

Preparation Method

MCF-7 cells were seeded in 96-well plates at a concentration of 1×105/mL and each well contained 100µL. After 24h, Fraxetin was added to 10, 20, 40 and 60µM final concentrations. The control group was treated without Fraxetin. The cells were incubated for 24 and 48h at 37℃ in 5% CO2 and then culture medium was changed.

Reaction Conditions

10-60μM; 24h, 48h

Applications

In MCF-7 cells, Fraxetin significantly inhibited cell proliferation in a concentration and time-dependent manner.
Animal experiment [2]:

Animal models

C57BL/6J mice

Preparation Method

C57BL/6J male 12-month-old mice maintained under natural lighting conditions in a temperature controlled (22 ± 3℃) room and acclimatized to laboratory environment for a minimum of 15 days before experimentation. Animals were segregated into two groups of 12 each: group a, control; and group b, experimental. All mice received a standard pelleted diet and had free access to food and water. The experimental group wastreated orally for 30 days with Fraxetin (25mg/kg) suspended in 10% (v/v) Tween-20 aqueous solution.

Dosage form

25mg/kg; po; 30d

Applications

Selenium-dependent glutathione reductase activity is significantly increased in the liver of Fraxetin treated mice.

References:
[1]. Liu G, Liu Z, Yan Y, et al. Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters. 2017 Dec 1; 14(6): 7374-7378.
[2]. MARTÍN?ARAGÓN SA, Benedí JM, Villar AM. Modifications on antioxidant capacity and lipid peroxidation in mice under fraxetin treatment. Journal of pharmacy and pharmacology. 1997 Jan; 49(1): 49-52.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
574-84-5
Synonyms
7,8-Dihydroxy-6-Methoxycoumarin
Chemical Name
7,8-dihydroxy-6-methoxychromen-2-one
SMILES
COC1=C(C(=C2C(=C1)C=CC(=O)O2)O)O
Molecular Formula
C10H8O5
Molecular Weight
208.17
Solubility
≥ 20.8mg/mL in DMSO
Storage
Store at 2-8°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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