Fusaric Acid (Synonyms: 5-Butylpicolinic Acid,5-Butylpyridine-2-Carboxylic Acid,NSC 135043) |
Catalog No.GC18061
One-Click Copy Product Info
|
Fusaric Acid, a picolinic acid derivative isolated from Fusarium heterosporium, is a fungal phytotoxin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 536-69-6
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Fusaric Acid, a picolinic acid derivative isolated from Fusarium heterosporium, is a fungal phytotoxin[1]. Fusaric Acid induces chloroplast and mitochondrial dysfunction, lipid peroxidation, protein damage and DNA fragmentation, causing wilting, necrotic spots on leaves, and reduction in root and root hair growth[2]. Fusaric Acid has been widely used in plant toxicity research to induce oxidative stress damage models in plant cells[3].
In vitro, Fusaric Acid treatment for 24 hours significantly inhibited the viability of human peripheral blood mononuclear cells (PBMCs) and Thp-1 cells, with IC50 values of 240.8μg/ml and 107.7μg/ml, respectively[4]. 200µg/ml Fusaric Acid incubation for 24 hours promoted cell death in HepG2 cells, decreased intracellular ATP levels, and downregulated the expression of mitochondrial stress response proteins SIRT3 and LON[5]. Incubation with 200µg/ml Fusaric Acid for 24 hours induced apoptosis in SNO cells, accompanied by increased Bax expression and decreased Bcl-2 expression[6].
In vivo, Fusaric acid treatment via oral administration at a dose of 100mg/kg/day for 14 days inhibited isoproterenol-induced cardiac hypertrophy and dysfunction, and alleviated cardiac fibrosis in mice[7]. Oral administration of Fusaric acid at a dose of 50mg/kg daily for 10 days reduced ATP levels, decreased the Akt signaling pathway, and increased the expression of glucose receptors in the brain tissues of mice[8].
References:
[1] López‐Díaz C, Rahjoo V, Sulyok M, et al. Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts[J]. Molecular Plant Pathology, 2018, 19(2): 440-453.
[2] Iqbal N, Czékus Z, Ördög A, et al. Fusaric acid-evoked oxidative stress affects plant defence system by inducing biochemical changes at subcellular level[J]. Plant Cell Reports, 2024, 43(1): 2.
[3] Singh V K, Upadhyay R S. Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L[J]. Botanical studies, 2014, 55(1): 66.
[4] Dhani S, Nagiah S, Naidoo D B, et al. Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells[J]. Scientific reports, 2017, 7(1): 3051.
[5] Abdul N S, Nagiah S, Chuturgoon A A. Fusaric acid induces mitochondrial stress in human hepatocellular carcinoma (HepG2) cells[J]. Toxicon, 2016, 119: 336-344.
[6] Devnarain N, Tiloke C, Nagiah S, et al. Fusaric acid induces oxidative stress and apoptosis in human cancerous oesophageal SNO cells[J]. Toxicon, 2017, 126: 4-11.
[7] Li X, Zhang Z L, Wang H F. Fusaric acid (FA) protects heart failure induced by isoproterenol (ISP) in mice through fibrosis prevention via TGF-β1/SMADs and PI3K/AKT signaling pathways[J]. Biomedicine & Pharmacotherapy, 2017, 93: 130-145.
[8] Dhani S, Ghazi T, Nagiah S, et al. Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue[J]. Food and Chemical Toxicology, 2020, 138: 111252.
| Cell experiment [1]: | |
|
Cell lines |
Thp-1 cells |
|
Preparation Method |
Thp-1 cells were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine, 1mM sodium pyruvate, 0.05mM β-mecaptoethanol, and 1% penicillin/streptomycin at 37°C in an incubator with 5% CO2. Cells were seeded in a 96-well plate at a density of 2×104 cells/well overnight and incubated with different concentrations of Fusaric Acid (0, 30, 60, 90, 120, 150, and 300µg/ml) for 24h, the cell proliferation was tested. |
|
Reaction Conditions |
0, 30, 60, 90, 120, 150, and 300µg/ml; 24h |
|
Applications |
Fusaric Acid treatment inhibited cell proliferation of Thp-1 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
|
Animal models |
Male C57BL/6 mice |
|
Preparation Method |
Male C57BL/6 mice (6 weeks old; 18-22g) were housed under standard conditions [temperature (22-24°C) and humidity (40-60%), a 12h light/dark cycle] with ad libitum access to food and normal drinking water. Fusaric Acid was administered orally via oral gavage once daily at a dose of 50mg/kg. Control groups received phosphate buffer saline (0.1M PBS; 0.25ml) (by oral gavage). At the end of the experimental periods (10 days), mice were euthanized by gas inhalation anaesthesia. Brain tissues were surgically removed for analysis. |
|
Dosage form |
50mg/kg/day; 10 days; p.o. |
|
Applications |
Fusaric Acid treatment dampened PI3K/Akt and AMPK signalling and increased the expression of GLUT receptors (1 and 4) in the mouse brain. |
References: [1] Dhani S, Nagiah S, Naidoo D B, et al. Fusaric Acid immunotoxicity and MAPK activation in normal peripheral blood mononuclear cells and Thp-1 cells[J]. Scientific reports, 2017, 7(1): 3051. [2] Dhani S, Ghazi T, Nagiah S, et al. Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue[J]. Food and Chemical Toxicology, 2020, 138: 111252. | |
| Cas No. | 536-69-6 | SDF | |
| Synonyms | 5-Butylpicolinic Acid,5-Butylpyridine-2-Carboxylic Acid,NSC 135043 | ||
| Chemical Name | 5-butyl-2-pyridinecarboxylic acid | ||
| Canonical SMILES | O=C(O)C1=CC=C(CCCC)C=N1 | ||
| Formula | C10H13NO2 | M.Wt | 179.2 |
| Solubility | DMF: 30 mg/ml,DMSO: 30 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 5.5804 mL | 27.9018 mL | 55.8036 mL |
| 5 mM | 1.1161 mL | 5.5804 mL | 11.1607 mL |
| 10 mM | 558 μL | 2.7902 mL | 5.5804 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 29 reference(s) in Google Scholar.)