Ac-Lys-OH is an important acetylated amino acid derivative, which is often used to increase the ligation/cyclization ratio in the reaction for generating peptide nitriles [1]. Ac-Lys-OH can be used in the amine titration experiment to confirm the presence of the active formyl group on the immobilized pyridoxal phosphate (PLP)[2]. Ac-Lys-OH has been widely used as a model molecule to study the steady-state kinetic parameters of the carboxylate oxygen exchange reaction[3].
References:
[1] Thoma B, Powner M W. Selective synthesis of lysine peptides and the prebiotically plausible synthesis of catalytically active diaminopropionic acid peptide nitriles in water[J]. Journal of the American Chemical Society, 2023, 145(5): 3121-3130.
[2] Lee K H, Distefano M D, Seelig B. Facile immobilization of pyridoxal 5′-phosphate using p-diazobenzoyl-derivatized Sepharose 4B[J]. Results in chemistry, 2023, 6: 101044.
[3] Hajkova D, Rao K C S, Miyagi M. pH dependency of the carboxyl oxygen exchange reaction catalyzed by lysyl endopeptidase and trypsin[J]. Journal of proteome research, 2006, 5(7): 1667-1673.