H-D-Val-Leu-Lys-pNA . 2 HCl (Synonyms: D-Val-Leu-Lys-p-nitroanilide, D-VLK-pNA, S 2251) |
Catalog No.GA22320
One-Click Copy Product Info
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H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62354-43-2
Sample solution is provided at 25 µL, 10mM.
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H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin [1]. H-D-Val-Leu-Lys-pNA . 2 HCl is catalytically bound and hydrolyzed by plasmin to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of plasminolytic activity[2]. H-D-Val-Leu-Lys-pNA . 2 HCl is a colorimetric substrate for the detection of protease activity, particularly plasmin has a high affinity for purified hypersensitive granuloma (gPA) and can be used to analyze macrophage activation in allergic granuloma of mice [3]. H-D-Val-Leu-Lys-pNA . 2 HCl has been widely used to identify extracellular proteases from micromycete[4].
References:
[1] Nagamatsu Y, Okamoto U, Tsuda Y, et al. Human leukocyte elastase-like proteinase purified by affinity chromatography with Suc-L-Tyr-D-Leu-D-Val-pNA, and its identification with human spleen fibrinolytic proteinase[J]. Thrombosis and haemostasis, 1984, 51(02): 243-247.
[2] Iwasaka M, Ueno S, Tsuda H. Effect of magnetic fields on the enzymatic activity of plasmin[C]//Proceedings of 16th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1994, 2: 762-763.
[3] Izaki S, Hibino T, Isozaki Y, et al. Plasminogen activator and plasminogen activator inhibitor associated with granulomatous inflammation: A study with murine leprosy[J]. Thrombosis and haemostasis, 1984, 52(06): 243-249.
[4] Stief T W, Richter A, Bünder R, et al. Functional Determination of Plasminin Arginine-stabilized Plasma[J]. Clinical and applied thrombosis/hemostasis, 2005, 11(3): 303-309.
| Cas No. | 62354-43-2 | SDF | |
| Synonyms | D-Val-Leu-Lys-p-nitroanilide, D-VLK-pNA, S 2251 | ||
| Formula | C23H38N6O5 · 2 HCl | M.Wt | 551.51 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8132 mL | 9.066 mL | 18.132 mL |
| 5 mM | 362.6 μL | 1.8132 mL | 3.6264 mL |
| 10 mM | 181.3 μL | 906.6 μL | 1.8132 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)