Jagged-1 (188-204) TFA |
Catalog No.GC36361
One-Click Copy Product Info
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Jagged-1 (188-204) TFA is a fragment of the Jagged-1 (JAG-1) protein, an agonist of the neurogenic site Notch homologous protein 1 (Notch1).
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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Jagged-1 (188-204) TFA is a fragment of the Jagged-1 (JAG-1) protein, an agonist of the neurogenic site Notch homologous protein 1 (Notch1)[1]. Jagged-1 is a Notch ligand that is highly expressed in cultured and primary multiple myeloma (MM) cells[2]. Jagged-1-mediated Notch activation can induce full maturation of human keratinocytes[3]. Jagged-1-mediated Notch activation can promote the differentiation of human limbal epithelial cells[4].
In vitro, treatment of human keratinocytes (KC) with Jagged-1 (188-204) TFA (40μM) for 72h induced the expression of loricrin and involucrin, and induced the cellular redistribution of Notch1, 3, and 4[3]. Jagged-1 (188-204) TFA (50μM) treatment of human pulmonary artery smooth muscle cells (PASMC) for 0-60min enhanced store-operated Ca2+ entry (SOCE) in the cells[5].
References:
[1] Chiappara G, Sciarrino S, Di Sano C, et al. Notch‐1 signaling activation sustains overexpression of interleukin 33 in the epithelium of nasal polyps[J]. Journal of Cellular Physiology, 2019, 234(4): 4582-4596.
[2] Jundt F, Pröbsting K S, Anagnostopoulos I, et al. Jagged1-induced Notch signaling drives proliferation of multiple myeloma cells[J]. Blood, 2004, 103(9): 3511-3515.
[3] Nickoloff B J, Qin J Z, Chaturvedi V, et al. Jagged-1 mediated activation of notch signaling induces complete maturation of human keratinocytes through NF-κB and PPARγ[J]. Cell Death & Differentiation, 2002, 9(8): 842-855.
[4] González S, Halabi M, Ju D, et al. Role of Jagged1-mediated Notch signaling activation in the differentiation and stratification of the human limbal epithelium[J]. Cells, 2020, 9(9): 1945.
[5] Yamamura H, Yamamura A, Ko E A, et al. Activation of Notch signaling by short-term treatment with Jagged-1 enhances store-operated Ca2+ entry in human pulmonary arterial smooth muscle cells[J]. American Journal of Physiology-Cell Physiology, 2014, 306(9): C871-C878.
| Cell experiment [1]: | |
Cell lines | Human pulmonary arterial smooth muscle cells (PASMC) |
Preparation Method | In human PASMCs, 10μM cyclopiazonic acid (CPA) was used to passively deplete Ca2+ from the sarcoplasmic reticulum (SR) to induce store-operated Ca2+ entry (SOCE). Cells were treated with 50μM Jagged-1 (188-204) TFA for 0, 5, 10, 30, and 60min, and changes in cytoplasmic free Ca2+ concentration caused by SOCE were recorded. |
Reaction Conditions | 50μM; 0-60min |
Applications | Short-term treatment with Jagged-1 (188-204) TFA enhances store-operated Ca2+ entry (SOCE) in human pulmonary arterial smooth muscle cells (PASMC). |
References: | |
| Cas No. | SDF | ||
| Formula | C95H128F3N25O28S3 | M.Wt | 2221.42 |
| Solubility | Water: 50 mg/mL (22.51 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 450.2 μL | 2.2508 mL | 4.5016 mL |
| 5 mM | 90 μL | 450.2 μL | 900.3 μL |
| 10 mM | 45 μL | 225.1 μL | 450.2 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)