Pro-xylane is a bioactive C-glycoside derived from Fagus sylvatica xylose. Pro-xylane stimulates the synthesis of glycosaminoglycans (GAGs) and hyaluronic acid to fill the extracellular matrix and lock in moisture, while strengthening the dermo-epidermal junction by promoting the synthesis of type IV and VII collagens. Pro-xylane can be used in studies related to skin anti-aging and chronic wound healing[1-4].
In vitro, epidermal keratinocytes and dermal fibroblasts were treated with Pro-xylane (1–3mM) for 48 hours. Pro-xylane significantly induced filaggrin expression and promoted α-smooth muscle actin generation, while inhibiting the secretion of the inflammatory factor prostaglandin E2[5]. Pro-xylane (0.3–3mM) was applied to dermal fibroblasts for 72 hours. Pro-xylane significantly stimulated the synthesis of sulfated glycosaminoglycans (GAGs)[6].
References:
[1] Dou Z, Hu PR, Song LL, et al. Extensive gene mining and facile engineering of a novel carbonyl reductase for asymmetric synthesis of anti-aging (S)-Pro-Xylane from d-xylose. Int J Biol Macromol. 2025 May;305(Pt 2):140976. doi: 10.1016/j.ijbiomac.2025.140976.
[2] Zhao Y, Li Y, Shang J, et al. Engineering a Carbonyl Reductase for High-Efficiency Synthesis of Optically Pure (S)-Pro-Xylane: An Alternative Synthetic Route. J Agric Food Chem. 2025 Apr 23;73(16):9759-9768.
[3] Jiang X, Peng S, Chen Y, et al. A Randomized, Investigator-Blinded, Split-Face, Controlled Trial Evaluating the Efficacy and Satisfaction of a Topical Product Containing Blueberry Extract and Pro-Xylane Combined With Micro-Focused Ultrasound for Anti-aging. J Cosmet Dermatol. 2025 Jul;24(7):e70281.
[4] Zang S, Chen J, Chevalier C, et al. Holistic investigation of the anti-wrinkle and repair efficacy of a facial cream enriched with C-xyloside. J Cosmet Dermatol. 2024 Dec;23(12):4017-4028.
[5] Ablon G, Paturi J, Juchaux F, et al. The Application of a Formulation Containing Hydroxypropyl Tetrahydropyrantriol and Niacinamide to Augment Anti-aging Benefits in a Double-blind, Randomized Nonablative Laser Treatment Study. J Clin Aesthet Dermatol. 2025 Dec;18(12):66-72.
[6] Cavezza A, Boulle C, Guéguiniat A, et al. Synthesis of Pro-Xylane: a new biologically active C-glycoside in aqueous media. Bioorg Med Chem Lett. 2009 Feb 1;19(3):845-9.