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SMCC (Synonyms: NSC 344483, Succinimidyl 4-(N-maleimidomethyl)cyclohexan-1-carboxylate)

Catalog No.GC38695

SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses.

Products are for research use only. Not for human use. We do not sell to patients.

SMCC Chemical Structure

Cas No.: 64987-85-5

Size Price Stock Qty
100mg
$32.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

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Background

SMCC is a protein crosslinker. SMCC-conjugated antigen coupled spleen cells to induce antigen-specific immune responses[1].

SMCC or sulfo-SMCC can use to conjugate keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). In the the conjugation system, 300 μl KLH or OVA (3 mg) and 30 μl SMCC or Sulfo-SMCC (the final concentration of 0.1 mM) are added in PBS with final volume of 3 ml[1].

Using the SMCC-conjugation method is able to induce potent immune responses to whole protein or peptide antigens by injection of antigen-coupled syngeneic splenic mononuclear cells in mice[1].

[1]. Guo Y, et al. Potent antigen-specific immune response induced by infusion of spleen cells coupled with succinimidyl-4-(N-maleimidomethyl cyclohexane)-1-carboxylate (SMCC) conjugated antigens. Int Immunopharmacol. 2016 Feb;31:158-68.

Chemical Properties

Cas No. 64987-85-5 SDF
Synonyms NSC 344483, Succinimidyl 4-(N-maleimidomethyl)cyclohexan-1-carboxylate
Canonical SMILES O=C(C1CCC(CN2C(C=CC2=O)=O)CC1)ON3C(CCC3=O)=O
Formula C16H18N2O6 M.Wt 334.32
Solubility DMSO: 25 mg/mL (74.78 mM) Storage 4°C, away from moisture and light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.9911 mL 14.9557 mL 29.9115 mL
5 mM 0.5982 mL 2.9911 mL 5.9823 mL
10 mM 0.2991 mL 1.4956 mL 2.9911 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 38 reference(s) in Google Scholar.)

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