5-Biotin-G-Monophosphate allows the incorporation of biotin at the 5' end of RNA and is particularly useful for the development of aptamers[1]. RNA aptamers with 5'-end biotinylation can be generated by in vitro 2'-F RNA transcription using 3mM 5-Biotin-G-Monophosphate[1]. 5-Biotin-G-Monophosphate is a modified nucleotide that incorporates biotin and is a versatile and reliable labeling reagent for the detection of nucleic acids, particularly in DNA amplification, sequencing, and hybridization applications[2]. 5-Biotin-G-Monophosphate is also an ideal tool for screening and analyzing enzyme activities that depend on nucleotide substrates[3].
References:
[1] Kahsai A W, Wisler J W, Lee J, et al. Conformationally selective RNA aptamers allosterically modulate the β2-adrenoceptor[J]. Nature chemical biology, 2016, 12(9): 709-716.
[2] Di Vito A, Reitano E, Poggi L, et al. Biotin oligonucleotide labeling reactions: A method to assess their effectiveness and reproducibility[J]. Analytical biochemistry, 2020, 593: 113590.
[3] Esposito C L, Autiero I, Sandomenico A, et al. Targeted systematic evolution of an RNA platform neutralizing DNMT1 function and controlling DNA methylation[J]. Nature Communications, 2023, 14(1): 99.