IF 45
PMID: 40436017 ↗Hyocholic Acid
Catalog No.: GC40717Purity:>97.00%Synonyms: γ-Muricholic Acid
Hyocholic acid (γ-Muricholic acid) is the major bile acid in pigs and other mammals and is also found in urine samples of patients with cholestasis.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 547-75-1
10mM (in 1mL DMSO)In Stock
$129.00
1
5mgIn Stock
$117.00
1
10mgIn Stock
$198.00
1
25mgIn Stock
$432.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $129.00 | In Stock | 1 | |
| 5mg | $117.00 | In Stock | 1 | |
| 10mg | $198.00 | In Stock | 1 | |
| 25mg | $432.00 | In Stock | 1 |
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Citations
1 publicationsPublications from researchers using this productDescription
Hyocholic acid (γ-Muricholic acid) is the major bile acid in pigs and other mammals and is also found in urine samples of patients with cholestasis[1]. Hyocholic acid promotes intracellular glucagon-like peptide-1 (GLP-1) secretion by activating G protein-coupled bile acid receptor (TGR5) and inhibiting farnesoid X receptor (FXR), thereby improving glucose homeostasis[2]. Hyocholic acid can be used as a novel biomarker for metabolic disorders and can resist type 2 diabetes[3].
In vitro, treatment of STC-1 and NCI-H716 cells with hyocholic acid (25, 50 μM) for 24 h upregulated GLP-1 protein secretion and proglucagon gene transcription in cells [4].
In vivo, oral treatment of diabetic mice with hyocholic acid (100 mg/kg) reduced blood glucose levels, increased fasting insulin levels, and upregulated serum GLP-1 levels through TGR5 and FXR signaling in vivo [4]. Oral treatment of nonalcoholic hepatitis mice with hyocholic acid (10, 100 mg/kg) for 16 weeks alleviated hepatic steatosis induced by a high-fat, high-cholesterol (HFHC) diet, reduced the production of lipid peroxides in a dose-dependent manner, and prevented hepatocyte apoptosis[5].
References:
[1] Lundell K, Wikvall K. Species-specific and age-dependent bile acid composition: aspects on CYP8B and CYP4A subfamilies in bile acid biosynthesis[J]. Current drug metabolism, 2008, 9(4): 323-331.
[2] Jia W, Rajani C, Zheng X, et al. Hyocholic acid and glycemic regulation: Comments on ‘Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism’[J]. Journal of Molecular Cell Biology, 2021, 13(6): 460-462.
[3] Zheng X, Chen T, Zhao A, et al. Hyocholic acid species as novel biomarkers for metabolic disorders[J]. Nature communications, 2021, 12(1): 1487.
[4] Zheng X, Chen T, Jiang R, et al. Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism[J]. Cell metabolism, 2021, 33(4): 791-803. e7.
[5] Xie Y, Shen F, He Y, et al. Gamma-muricholic acid inhibits nonalcoholic steatohepatitis: Abolishment of steatosis-dependent peroxidative impairment by FXR/SHP/LXRα/FASN signaling[J]. Nutrients, 2023, 15(5): 1255.
Protocol
| Cell experiment [1]: | |
Cell lines | STC-1 and NCI-H716 cells |
Preparation Method | Cells were treated with different concentrations (5, 25, and 50μM) of Hyocholic Acid with 24h treatment for determination of GLP-1 secretion and proglucagon transcription. |
Reaction Conditions | 5, 25, 50μM; 24h |
Applications | Hyocholic Acid upregulates GLP-1 protein secretion and proglucagon gene transcription in STC-1 and NCI-H716 cells. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice |
Preparation Method | C57BL/6 mice were randomized into the normal control (NC), NASH, NASH+vehicle, NASH+10mg/kg Hyocholic Acid, and NASH+100mg/kg Hyocholic Acid groups, respectively. Except for those in the NC group with a normal diet, all the mice were exposed to the high-fat high-cholesterol(HFHC) diet (2% cholesterol, 10% lard, and 88% normal diet) for 16 weeks. |
Dosage form | 10、100mg/kg; p.o. |
Applications | Hyocholic Acid attenuated rodent liver steatosis induced by the HFHC diet. Hyocholic Acid treatment dose-dependently reduced the production of lipid peroxides. Hyocholic Acid treatment dose-dependently prevented hepatocytes from the apoptotic process. |
References: [1]Zheng X, Chen T, Jiang R, et al. Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism[J]. Cell metabolism, 2021, 33(4): 791-803. e7. [2]Xie Y, Shen F, He Y, et al. Gamma-muricholic acid inhibits nonalcoholic steatohepatitis: Abolishment of steatosis-dependent peroxidative impairment by FXR/SHP/LXRα/FASN signaling[J]. Nutrients, 2023, 15(5): 1255. | |
Product Documents
Purity:>97.00%Appearance:A solid
Chemical Properties
CAS No.
547-75-1
Synonyms
γ-Muricholic Acid
Chemical Name
(5β)-3α,6α,7α-trihydroxy-cholan-24-oic acid
SMILES
C[C@H](CCC(O)=O)[C@@]1([H])CC[C@@]2([H])[C@]3([H])[C@H](O)[C@H](O)[C@]4([H])C[C@H](O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C
Molecular Formula
C24H40O5
Molecular Weight
408.6
Solubility
30 mg/ml in DMF, 20 mg/ml in DMSO, 20 mg/ml in Ethanol
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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