INCA-6 (Synonyms: Triptycene-1,4-quinone) |
Catalog No.GC16117
One-Click Copy Product Info
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A cell-permeant inhibitor of NFAT signaling
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3519-82-2
Sample solution is provided at 25 µL, 10mM.
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INCA-6 is a selective inhibitor of transcription factor NFAT with Kd value of 0.8 mM in vitro[1].
NFAT (Nuclear factor of activated T-cells) is a general name which is applied to a family of transcription factors that are important in immune response. They are expressed in most immune system cells. Calcium signaling plays an important role in NFAT activation. Calmodulin activates calcineurin (CN) which is a serine/threonine phosphatase, then dephosphorylates the amino termini of NFAT proteins resulting a conformational change. NFAT will transport into nuclear. NFAT proteins will cooperate with other nuclear resident transcription factors due to that they have weak DNA-binding capacity. NFAT proteins have weak DNA-binding capacity NFAT transcription factors are specifically implicated in the process of cell motility and are related to breast cancer.[2]
INCA-6 treatment significant blocked translocation of nuclear factor of activated T-cells c1. INCA-6 effectively blocked the stimulation of vascular endothelial growth factor stimulation at 1.0 μM. INCA-6 significantly tube formation at 1.0μM in human retinal microvascular endothelial cells[1]. INCA-6 at 50 μM inhibited ATP-induced activation of NFATc1 in MG-5 cells.[3] In BV-2 cells, INCA-6 at 10
μM significantly inhibited ATP-induced CXCL2 expression.[4] INCA-6 reversed the proliferative effect of E2.[5]
References:
[1]. Bretz CA, Savage S, Capozzi M, Penn JS: The role of the NFAT signaling pathway in retinal neovascularization. Invest Ophthalmol Vis Sci 2013, 54(10):7020-7027.
[2]. Crabtree GR, Olson EN: NFAT signaling: choreographing the social lives of cells. Cell 2002, 109 Suppl:S67-79.
[3]. Kataoka A, Tozaki-Saitoh H, Koga Y, Tsuda M, Inoue K: Activation of P2X7 receptors induces CCL3 production in microglial cells through transcription factor NFAT. J Neurochem 2009, 108(1):115-125.
[4]. Shiratori M, Tozaki-Saitoh H, Yoshitake M, Tsuda M, Inoue K: P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways. J Neurochem 2010, 114(3):810-819.
[5]. Wong CK, So WY, Law SK, Leung FP, Yau KL, Yao X, Huang Y, Li X, Tsang SY: Estrogen controls embryonic stem cell proliferation via store-operated calcium entry and the nuclear factor of activated T-cells (NFAT). J Cell Physiol 2012, 227(6):2519-2530.
| Cas No. | 3519-82-2 | SDF | |
| Synonyms | Triptycene-1,4-quinone | ||
| Chemical Name | (9s,10s)-9,10-[1,2]benzenoanthracene-13,16(9H,10H)-dione | ||
| Canonical SMILES | O=C(C=CC1=O)C2=C1[C@@H]3C(C=CC=C4)=C4[C@H]2C5=C3C=CC=C5 | ||
| Formula | C20H12O2 | M.Wt | 284.31 |
| Solubility | DMF: 20 mg/mL,DMSO: 2 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.5173 mL | 17.5864 mL | 35.1729 mL |
| 5 mM | 703.5 μL | 3.5173 mL | 7.0346 mL |
| 10 mM | 351.7 μL | 1.7586 mL | 3.5173 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)