IR 780 |
Catalog No.GC48396
One-Click Copy Product Info
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IR 780 is a heptacyanine fluorescent probe for in vivo imaging of tumor cells with a large absorption spectrum of 750-830nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 207399-07-3
Sample solution is provided at 25 µL, 10mM.
IR 780 is a heptacyanine fluorescent probe for in vivo imaging of tumor cells with a large absorption spectrum of 750-830nm.It is also a powerful and stable nanotheranostic agent[1,2]. The hydrophobic properties of IR-780 enable it to be encapsulated in the bilayer of liposome with high encapsulation efficiency. IR-780 has high singlet oxygen (1O2) quantum yield, photostability and fluorescence intensity [3], For IR-780, the 1O2 quantum yield could reach 0.127[4].
IR-780(0-160 µM;24h) induced dose-dependent inhibition of PC-3, LNCaP, and RWPE-1 cells proliferation [5].IR-780 can be efficiently taken up by RCC cells through the organic anion-transporting polypeptide (OATP) superfamily transporters, which enables the diagnosis of even small lesions through tumor tissue-specific imaging [6]. Uptake of IR-780(4 µM, 10 µM or 16 µM ;1-4h) is dose and time-dependent in 4T1 cancer cells. When these cancer cells were treated with IR-780 and US irradiation, survival was significantly reduced and necrotic/apoptotic cells increased [7].
IR-780(0.334 mg/kg and 3.334 mg/kg; i.p.; daily for 1 month) (10 times imaging dose) staining for 1 month had no significant effect on physical activity weight and histology of BABL/C mice. High signal-to-background ratios of xenografts were achieved in athymic nude mice models (IR-780 dye application in prostate cancer detection) 24 h after NIRF dyes administration [6]. Using intracranial glioma xenograft in nude mice and administration of ILs (including IR-780) by convection enhanced delivery (CED) to overcome blood-brain barrier, liposomal IR-780(0.8 g/L IR-780) could be specifically delivered to the brain tumor [8].
References:
[1]. Li S, Zhou S, et,al. Exceptionally High Payload of the IR780 Iodide on Folic Acid-Functionalized Graphene Quantum Dots for Targeted Photothermal Therapy. ACS Appl Mater Interfaces. 2017 Jul 12;9(27):22332-22341. doi: 10.1021/acsami.7b07267. Epub 2017 Jun 28. Erratum in: ACS Appl Mater Interfaces. 2020 Jun 17;12(24):27819-27820. PMID: 28643511.
[2]. Wang K, Zhang Y, et,al. Self-assembled IR780-loaded transferrin nanoparticles as an imaging, targeting and PDT/PTT agent for cancer therapy. Sci Rep. 2016 Jun 6;6:27421. doi: 10.1038/srep27421. PMID: 27263444; PMCID: PMC4899881.
[3]. Zhang L, Wang D, Yang K, Sheng D, Tan B, Wang Z, Ran H, Yi H, Zhong Y, Lin H, Chen Y. Mitochondria-Targeted Artificial "Nano-RBCs" for Amplified Synergistic Cancer Phototherapy by a Single NIR Irradiation. Adv Sci (Weinh). 2018 May 21;5(8):1800049. doi: 10.1002/advs.201800049. PMID: 30128231; PMCID: PMC6097143.
[4]. Ren H, Liu J, et,al. Relighting Photosensitizers by Synergistic Integration of Albumin and Perfluorocarbon for Enhanced Photodynamic Therapy. ACS Appl Mater Interfaces. 2017 Feb 1;9(4):3463-3473. doi: 10.1021/acsami.6b14885. Epub 2017 Jan 18. PMID: 28067039.
[5]. Yi X, Yan F, et,al. IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586.
[6]. Yang X, Shao C, et,al. Optical imaging of kidney cancer with novel near infrared heptamethine carbocyanine fluorescent dyes. J Urol. 2013 Feb;189(2):702-710. doi: 10.1016/j.juro.2012.09.056. Epub 2012 Sep 20. PMID: 23000848; PMCID: PMC4120709.
[7]. Li Y, Zhou Q, et,al. IR-780 Dye as a Sonosensitizer for Sonodynamic Therapy of Breast Tumor. Sci Rep. 2016 May 13;6:25968. doi: 10.1038/srep25968. PMID: 27174006; PMCID: PMC4865802.
[8]. Lu YJ, S AT, et,al. Liposomal IR-780 as a Highly Stable Nanotheranostic Agent for Improved Photothermal/Photodynamic Therapy of Brain Tumors by Convection-Enhanced Delivery. Cancers (Basel). 2021 Jul 22;13(15):3690. doi: 10.3390/cancers13153690. PMID: 34359590; PMCID: PMC8345063.
| Cell experiment [1]: | |
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Cell lines |
PC-3, LNCaP, and RWPE-1 cells |
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Preparation Method |
Cells were seeded in 96-well plates (5x103 cells/well) and continued to grow 12 h for cell adhesion. After 24-h incubation with IR-780, cells were assessed for the inhibition of cell proliferation after 24 h exposure using the CCK-8 assay kit. |
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Reaction Conditions |
0-160µM;24h |
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Applications |
IR-780 induced dose-dependent inhibition of cell proliferation. |
| Animal experiment [2]: | |
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Animal models |
BALB/C mice |
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Preparation Method |
PC-3 cells were implanted subcutaneously into 3 athymic nude mice, besides, 3 athymic nude mice without inoculation as normal controls. When tumor sizes reached about 5-10 mm in diameter. IR-780 dye was injected i.p. at a dose of 0.334 mg/kg. The mice were anesthetized 24 h after IR-780 dye injection by 2% isoflurane in 100% oxygen with a delivery rate of 1.5 L/min. The whole-body investigate near-infrared fluorescence (NIRF) imaging of normal and tumor-loaded mice was taken using a IVIS Lumina II imaging station. |
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Dosage form |
0.334 mg/kg; i.p. |
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Applications |
High signal-to-background ratios of xenografts were achieved in athymic nude mice models(IR-780 dye application in prostate cancer detection)24 h after NIRF dyes administration. |
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References: [1]. Yi X, Yan F, et,al . IR-780 dye for near-infrared fluorescence imaging in prostate cancer. Med Sci Monit. 2015 Feb 16;21:511-7. doi: 10.12659/MSM.892437. PMID: 25686161; PMCID: PMC4335586. |
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| Cas No. | 207399-07-3 | SDF | |
| Canonical SMILES | ClC1=C(/C=C/C2=[N+](CCC)C3=C(C2(C)C)C=CC=C3)CCC/C1=C\C=C4N(CCC)C5=C(C/4(C)C)C=CC=C5.[I-] | ||
| Formula | C36H44ClN2•I | M.Wt | 667.1 |
| Solubility | Chloroform: 10 mg/ml,DMF: slightly soluble,DMSO: slightly soluble,Ethanol: slightly soluble | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.499 mL | 7.4951 mL | 14.9903 mL |
| 5 mM | 299.8 μL | 1.499 mL | 2.9981 mL |
| 10 mM | 149.9 μL | 749.5 μL | 1.499 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)