Baicalin (Synonyms: Baicalein 7-glucuronide) |
カタログ番号GN10018
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バイカリン(Baicalin)はフラボノイド配糖体であり、アロステリックカルニチンパルミトイルトランスフェラーゼ1（CPT1）活性化剤でもある。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21967-41-9
Sample solution is provided at 25 µL, 10mM.
バイカリン(Baicalin)はフラボノイド配糖体であり、アロステリックカルニチンパルミトイルトランスフェラーゼ1（CPT1）活性化剤でもある[1]。バイカリンは、ヒト免疫不全ウイルスタイプ1(HIV-1)の複製を阻害できる[2]。バイカリンは、NF-κBの発見を減らし、重要な癌シグナル経路の制御を仲介できる[3]。
試験管内実験では、バイカリン(0.005-0.5nM)でRAW264.7細胞を処理した結果、LPS誘発性細胞の増殖を阻害し、細胞内接着分子-1(ICAM-1)、単球走化性タンパク質-1(MCP-1)とシクロオキシゲナーゼ-2(Cox-2)タンパク質の発見を減らした[4]。バイカリン(100μM)でHK-2細胞を1時間事前処理した結果、H2O2刺激後の細胞生存率を促進でき、酸化ストレスを減らし、カスパーゼ-3の活性と細胞のアポトーシスを阻害できる[5]。
生体内実験では、バイカリン(10, 100 mg/kg)は腎虚血再灌流障害(IRI)のラットに腹腔内注射した結果、酸化ストレスと組織学的損傷を減らし、腎機能を改善し、炎症誘発反応と尿細管アポトーシスを阻害し、TLR2, TLR4, MyD88, p-NF-κBとp-IκBタンパク質の発見とカスパーゼ-3の活性を減らし、Bcl-2/Bax比を増やした[6]。糖尿病性腎症(DN)のマウスにバイカリン(400 mg/kg)を腹腔内注射した結果、糖尿病の症状、タンパク尿、腎臓の組織病理学的変化と細胞のアポトーシスを効果的に改善し、Erk1/2, JNKとP38MAPKシグナル経路などの典型的な炎症促進シグナル経路MAPKファミリーの活性を阻害した[7]。
References:
[1] Dai J, Liang K, Zhao S, et al. Chemoproteomics reveals baicalin activates hepatic CPT1 to ameliorate diet-induced obesity and hepatic steatosis[J]. Proceedings of the National Academy of Sciences, 2018, 115(26): E5896-E5905.
[2] Kitamura K, Honda M, Yoshizaki H, et al. Baicalin, an inhibitor of HIV-1 production in vitro[J]. Antiviral research, 1998, 37(2): 131-140.
[3] Yu X, Liu Y, Wang Y, et al. Baicalein induces cervical cancer apoptosis through the NF-κB signaling pathway[J]. Molecular Medicine Reports, 2018, 17(4): 5088-5094.
[4] Cui L, Feng L, Zhang Z H, et al. The anti-inflammation effect of baicalin on experimental colitis through inhibiting TLR4/NF-κB pathway activation[J]. International Immunopharmacology, 2014, 23(1): 294-303.
[5] Lin M, Li L, Zhang Y, et al. Baicalin ameliorates H2O2 induced cytotoxicity in HK-2 cells through the inhibition of ER stress and the activation of Nrf2 signaling[J]. International journal of molecular sciences, 2014, 15(7): 12507-12522.
[6] Lin M, Li L, Li L, et al. The protective effect of baicalin against renal ischemia-reperfusion injury through inhibition of inflammation and apoptosis[J]. BMC complementary and alternative medicine, 2014, 14: 1-9.
[7] Ma L, Wu F, Shao Q, et al. Baicalin alleviates oxidative stress and inflammation in diabetic nephropathy via Nrf2 and MAPK signaling pathway[J]. Drug design, development and therapy, 2021: 3207-3221.
| 細胞実験[1]: | |
細胞株 | RAW264.7細胞 |
準備方法 | 0.005-0.5nMのバイカリンとLPS(1 μg/ml)で細胞を処理した。10μl(5mg/ml)のMTT溶液は各ウェルに4時間添加した。培養の末、100μlのDMSOは培地除去の10分後に添加した。 |
反応条件 | 0.005-0.5nM; 4時間 |
アプリケーション | 1μg/mlのLPSに暴露することは、細胞の増殖を増やした。バイカリンはLPS誘発性RAW264.7細胞の増殖を阻害した。 |
| 動物実験 [2]: | |
動物モデル | 雄のWistarラット |
準備方法 | ラットは6匹ずつ五つのグループにランダムに分け、左腎動脈を45分間遮断し、右腎摘出術を実施することで腎臓IRIを誘発した。生理食塩水で処理された動物は腎臓遮断の30分前に、1mL 0.9%無菌NaClを腹腔内注射した。バイカリンで処理されたラットは腎臓遮断の30分前に、無菌の生理食塩水で体重1kgあたり1, 10, または100 mgに希釈されたバイカリンを腹腔内注射した。 |
投与形態 | 1、10、100mg/kg；腹腔内注射 |
アプリケーション | バイカリンの投与は、酸化ストレスと組織学的損傷を減らし、腎機能を改善し、炎症促進反応と尿細管アポトーシスをも阻害した。 |
References: | |
| Cas No. | 21967-41-9 | SDF | |
| 同義語 | Baicalein 7-glucuronide | ||
| Chemical Name | (2S,3S,4S,5R,6S)-6-(5,6-dihydroxy-4-oxo-2-phenylchromen-7-yl)oxy-3,4,5-trihydroxyoxane-2-carboxylic acid | ||
| Canonical SMILES | C1=CC=C(C=C1)C2=CC(=O)C3=C(C(=C(C=C3O2)OC4C(C(C(C(O4)C(=O)O)O)O)O)O)O | ||
| Formula | C21H18O11 | M.Wt | 446.37 |
| 溶解度 | DMSO: ≥ 100 mg/mL (224.03 mM） | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.2403 mL | 11.2015 mL | 22.4029 mL |
| 5 mM | 448.1 μL | 2.2403 mL | 4.4806 mL |
| 10 mM | 224 μL | 1.1201 mL | 2.2403 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Related Biological Data
Baicalin prevented ΔΨm loss and mitochondrial ROS generation that BLM-induced in MLE-12, 5-HD blocked the protective effect. (A) MitoTracker (red) probe indicated the change of mitochondrial membrane potential changes in MLE-12 cells.
For experiments, cells were seeded in plates and grown to 60%− 70% confluency before they were treated with 5-Hydroxydecanoate sodium was (100μM) for 30min, diazoxide (100μM) or baicalin (90μM，GLPBIO, USA) for 30min.
Toxicology (2023): 153638. PMID: 37783230 IF: 4.5003 -
Related Biological Data
Baicalin inhibited expression of FTH1 in OSCC cells. (I, J) Western blot analysis of FTH1 expression changes after overexpression and baicalin treatment.
The CCK8 assay was used to detect the cell viability of Cal27 and SCC25 cells treated with baicalin (GLPBIO, USA) at 0, 5, 10, 20, 4, and 60μM for 24h.
The Journal of Gene Medicine 26.2 (2024): e3669. PMID: 38380717 IF: 3.5
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)