Amyloid Beta-Peptide (12-28) (human) |
| Catalog No.GP10049 |
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 107015-83-8
Sample solution is provided at 25 µL, 10mM.
GlpBio Products Cited In Reputable Papers
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Product Documents
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Protocol
| Cell experiment [1]: | |
|
Cell lines |
PC12 cells |
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Preparation Method |
Two days after plating cell were treated with different concentrations of aged β12-28 peptide. After 22 h, MTT (5 mg/ml) was added and incubation was continued for a further2 h. Cells were lysed and after 24 h at 37°C, colorimetric determination of MTT reduction was made at 550 nm. |
|
Reaction Conditions |
0.001, 0.1, 10, 40 µM for 22 hours |
|
Applications |
Freshly pre-pared solutions of β12 - 28 were non-toxic as expected, while aged solutions produce generally significant decreases in the MTT mitochondrial reduction by PC12 cells. |
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References: [1]: Rabanal, F., Tusell, J. M., Sastre, L., et al. (2002) Structural, kinetic and cytotox-icity aspects of 12-28 β amyloid protein fragment: A reappraisal. J. Pept. Sci. 8,578-588. |
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| Cas No. | 107015-83-8 | SDF | |
| Formula | C89H135N25O25 | M.Wt | 1955.2 |
| Solubility | ≥195.5mg/mL in DMSO | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Complete Stock Solution Preparation Table
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.5115 mL | 2.5573 mL | 5.1146 mL |
| 5 mM | 0.1023 mL | 0.5115 mL | 1.0229 mL |
| 10 mM | 0.0511 mL | 0.2557 mL | 0.5115 mL |
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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