C12E8 (Synonyms: Octaethylene Glycol Monododecyl Ether) |
Catalog No.GC43020
One-Click Copy Product Info
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C12E8은 비이온성 계면활성제로 풀네임은 폴리옥시에틸렌 글리콜 계면활성제이고 폴리옥시에틸렌 에터 계면활성제에 속한다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3055-98-9
Sample solution is provided at 25 µL, 10mM.
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C12E8은 비이온성 계면활성제로 풀네임은 폴리옥시에틸렌 글리콜 계면활성제이고 폴리옥시에틸렌 에터 계면활성제에 속한다. C12E8은 인지질 베스틸 막의 곡률과 형태 변화를 농도 의존적으로 조절하고 낮은 농도에서는 자연적인 형태 변화를 촉진하고 높은 농도에서는 비정상적인 변형을 유도해서 최종적으로 베스틸의 분해를 초래한다[2-3].
DC3F 세포에서 C12E8(0.05mg/mL; 45분)은 평면 지질 이중층에서 불가역적 전기 투과를 위한 전압을 감소시킨다[4]. 적혈구에서 C12E8(44μM; 60분)으로 처리된 적혈구 단면에서 중앙부가 압축되고 주변부가 구형인 큰 평평한 invaginations/endovesicles가 발견되었다[5].
References:
[1]. Ruiz CC, Molina-Bolívar JA. Characterization of mixed non-ionic surfactants n-octyl-β-d-thioglucoside and octaethylene–glycol monododecyl ether: Micellization and microstructure. Journal of colloid and interface science. 2011 Sep 1; 361(1): 178-185.
[2]. Mavcic B, Babnik B, Iglic A, et al. Shape transformation of giant phospholipid vesicles at high concentrations of C12E8. Bioelectrochemistry. 2004 Jun 1; 63(1-2): 183-187.
[3]. Guo Y. Surface interactions between poly (ethylene glycol) and phospholipid bilayers: Effects on aggregation and fusion. State University of New York at Buffalo; 1993.
[4]. Kanduser M, Fosnaric M, Sentjurc M, et al. Effect of surfactant polyoxyethylene glycol (C12E8) on electroporation of cell line DC3F. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2003 Mar 12; 214(1-3): 205-217.
[5]. Bobrowska-Hägerstrand M, Kralj-Iglič V, Iglič A, et al. Torocyte membrane endovesicles induced by octaethyleneglycol dodecylether in human erythrocytes. Biophysical journal. 1999 Dec 1; 77(6): 3356-3362.
| 세포 실험 [1]: | |
세포 라인 | DC3F 세포 |
제조 방법 | 소생된 세포는 37℃에서 45분 동안 배양되었습니다. 처리된 세포에는 0.05mg/mL C12E8가 첨가되었고 C12E8가 녹아 있는 배지가 처리되지 않은 세포에 첨가되었습니다. 전자자기 공명법(EPR)으로 막 유동성을 측정하였고 지방성 물질로 막 지질 이중층에 주로 결합하는 5-doxyl palmitate (MeFASL)의 스핀 프로브를 사용했습니다. 60밀리리터의 0.1mM MeFASL 에탄올 용액을 유리관에 넣은 후 회전 증발기로 에탄올을 증발시켰습니다. 유리관 벽에 형성된 MeFASL막에 20×10⁶개의 세포를 포함하는 1mL의 세포 소생된 용액을 넣고 흔들면서 15분 동안 배양했습니다. 그 후 세포 소생된 용액을 원심 분리하고 침전물을 유리 모세관에 넣어 EPR 측정을 위해 사용했습니다. 4℃에서의 EPR 스펙트럼에서 차수 파라미터를 반영하는 최대 초미세 분할 상수 2AII를 측정했습니다. |
반응 조건 | 0.05mg/mL; 45분 동안 |
응용 분야 | C12E8은 평면 지질 이중층에서 비가역적인 전기화를 위한 전압을 감소시킵니다. |
References: | |
| Cas No. | 3055-98-9 | SDF | |
| Synonyms | Octaethylene Glycol Monododecyl Ether | ||
| Canonical SMILES | CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCO | ||
| Formula | C28H58O9 | M.Wt | 538.8 |
| Solubility | DMF: 30 mg/ml,DMSO: 15 mg/ml,Ethanol: 30 mg/ml,PBS (pH 7.2): 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.856 mL | 9.2799 mL | 18.5598 mL |
| 5 mM | 371.2 μL | 1.856 mL | 3.712 mL |
| 10 mM | 185.6 μL | 928 μL | 1.856 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: An oil
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)