DL-threo-β-Hydroxyaspartic Acid (Synonyms: DL-THA, DL-threo-3-Hydroxyaspartic Acid) |
Catalog No.GC49829
One-Click Copy Product Info
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An EAAT inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 4294-45-5
Sample solution is provided at 25 µL, 10mM.
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DL-threo-β-Hydroxyaspartic acid is an inhibitor of excitatory amino acid transporters (EAATs).1,2 It inhibits glutamate uptake in COS-1 cells expressing human EAAT1 or EAAT2 (IC50s = 96 and 31 µM, respectively) as well as inward currents induced by L-aspartate in EAAT4-expressing Xenopus oocytes (Ki = 0.6 µM) and by L-glutamate in EAAT5-expressing oocytes (Ki = 2.5 µM) voltage-clamped at -60 mV. Intrathecal administration of DL-threo-β-hydroxyaspartic acid (7.5 and 15 µg/animal) reduces the number of flinches and shakes in the second phase of the formalin test in rats.3
1.Shimamoto, K., Lebrun, B., Yasuda-Kamatani, Y., et al.??-threo-β-benzyloxyaspartate, a potent blocker of excitatory amino acid transporters.Mol. Pharmacol.53(2)195-201(1998) 2.Shigeri, Y., Shimamoto, K., Yasuda-Kamatani, Y., et al.Effects of threo-β-hydroxyaspartate derivatives on excitatory amino acid transporters (EAAT4 and EAAT5)J. Neurochem.79(2)297-302(2001) 3.Yaster, M., Guan, X., Petralia, R.S., et al.Effect of inhibition of spinal cord glutamate transporters on inflammatory pain induced by formalin and complete Freund’s adjuvantAnesthesiology114(2)412-423(2011)
| Cas No. | 4294-45-5 | SDF | |
| Synonyms | DL-THA, DL-threo-3-Hydroxyaspartic Acid | ||
| Canonical SMILES | OC([C@@H](O)[C@H](N)C(O)=O)=O | ||
| Formula | C4H7NO5 | M.Wt | 149.1 |
| Solubility | Acetonitrile: Slightly soluble,Chloroform: Slightly soluble,DMF: Slightly soluble,DMSO: Slightly soluble,Ethanol: Slightly soluble,PBS (pH 7.2): Slightly soluble,Water: Slightly soluble | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7069 mL | 33.5345 mL | 67.0691 mL |
| 5 mM | 1.3414 mL | 6.7069 mL | 13.4138 mL |
| 10 mM | 670.7 μL | 3.3535 mL | 6.7069 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)