Iristectorin B |
Catalog No.GC36331
One-Click Copy Product Info
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Iristectorin B는 항암 효과가 있는 이리스 테크토룸에서 유래한 천연 이소플라본이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 94396-09-5
Sample solution is provided at 25 µL, 10mM.
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Iristectorin B는 항암 효과가 있는 이리스 테크토룸에서 유래한 천연 이소플라본이다. Iristectorin B는 지방다당류(LPS)에 의해 유도된 산화질소(NO) 생성을 억제해서 항염증 활성을 발휘할 수 있다[2]. Iristectorin B는 관련 화합물의 추출 및 분리를 위한 이온성 액체 초음파 보조 추출 방법을 개발하기 위한 내부 기준물질로 일반적으로 사용된다 [3].
체외 실험에서 Iristectorin B (25 µM) 처리 24시간은 포도당 산소 결핍/재산소화(OGD/R) 처리 후 PC12 세포의 생존율을 현저하게 증가시키고 세포 사멸을 감소시켜서 TFR1、HMOX1 및 SLC3A2의 발현을 현저하게 감소시켰다[4].
References:
[1] Ruan L J, Yan B X, Wu Y Q, et al. Integrated Chemometric and Spectrum-effect analysis discriminates Belamcanda chinensis and Iris tectorum in treating A549 lung Cancer cells[J]. Journal of Chromatography B, 2025: 124875.
[2] Gao B, Ma Y, Zhang L, et al. Identification and characterization of the chemical components of Iris tectorum Maxim. and evaluation of their nitric oxide inhibitory activity[J]. Rapid communications in mass spectrometry, 2021, 35(1): e8959.
[3] Sun Y, Li W, Wang J. Ionic liquid based ultrasonic assisted extraction of isoflavones from Iris tectorum Maxim and subsequently separation and purification by high-speed counter-current chromatography[J]. Journal of Chromatography B, 2011, 879(13-14): 975-980.
[4] Zheng M, Zhou M, Lu T, et al. TMT and PRM Based Quantitative Proteomics to Explore the Protective Role and Mechanism of Iristectorin B in Stroke[J]. International Journal of Molecular Sciences, 2023, 24(20): 15195.
| 세포 실험 [1]: | |||
세포 라인 | PC12 세포 | ||
제조 방법 | PC12 세포는 10% 태아소 혈청(FBS) 및 1% 페니실린/스트렙토마이신이 첨가된 고포도 DMEM 배양지에서 37℃、5% CO₂ 조건 하에서 배양하였습니다. 10 mM Na₂S₂O₄가 포함된 무당 EBSS 용액 100μl을 첨가해서 2시간 배양하였습니다. 세포를 정상 배지로 교체해서 재산소화한 후 24시간 계속 배양하고 허혈성 뇌손상을 모사하는 OGD/R 손상 모델을 구축하였습니다. OGD/R 후 Iristectorin B 6.25µM、12.5µM 및 25µM이 포함된 정상 배양지를 보충해서 24시간 처리하였습니다. 이후 세포 생존율을 분석하였습니다. | ||
반응 조건 | 6.25µM, 12.5µM 및 25µM; 24 시간 동안 | ||
응용 분야 | Iristectorin B 처리는 OGD/R 손상 후 PC12 세포의 세포 생존율을 증가시켰습니다. | ||
| Cas No. | 94396-09-5 | SDF | |
| Canonical SMILES | O=C1C(C2=CC=C(O)C(OC)=C2)=COC3=CC(O[C@@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=C(OC)C(O)=C13 | ||
| Formula | C23H24O12 | M.Wt | 492.43 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0307 mL | 10.1537 mL | 20.3075 mL |
| 5 mM | 406.1 μL | 2.0307 mL | 4.0615 mL |
| 10 mM | 203.1 μL | 1.0154 mL | 2.0307 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)