ML367 |
| Catalog No.GC36630 |
ML367은 ATPase 계열 AAA 도메인 함유 단백질 5(ATAD5) 안정화의 강력한 억제제이며 마이크로몰 억제 활성이 낮은 프로브 분자로 작용합니다. ML367은 DNA 복구 경로를 차단하고 UV 조사에 대한 반응으로 RPA32-인산화 및 CHK1-인산화를 포함한 일반적인 DNA 손상 반응을 억제합니다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 381168-77-0
Sample solution is provided at 25 µL, 10mM.
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ML367 is a potent inhibitor of ATPase family AAA domain-containing protein 5 (ATAD5) stabilization, acts as a probe molecule that has low micromolar inhibitory activity. ML367 blocks DNA repair pathways, suppresses general DNA damage responses including RPA32-phosphorylation and CHK1-phosphorylation in response to UV irradiation[1]. ATAD5[1]
ML367 (0-40 μM, 16 hours) inhibits FLAG-ATAD5 stabilization in HEK293T cells in the presence or absence of 20 μM 5-FUrd[1]. Western Blot Analysis[1] Cell Line: HEK293T cells
[1]. Rohde JM, et al. Discovery of ML367, inhibitor of ATAD5 stabilization. Probe Reports from the NIH Molecular Libraries Program.
| Cas No. | 381168-77-0 | SDF | |
| Canonical SMILES | FC1=CC(NC2=NC(C3=CC=NC=C3)=NC4=CC=CC=C42)=CC=C1F | ||
| Formula | C19H12F2N4 | M.Wt | 334.32 |
| Solubility | DMSO: ≥ 125 mg/mL (373.89 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.9911 mL | 14.9557 mL | 29.9115 mL |
| 5 mM | 598.2 μL | 2.9911 mL | 5.9823 mL |
| 10 mM | 299.1 μL | 1.4956 mL | 2.9911 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)
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