Ethyl glucuronide |
Catalog No.GC38593
One-Click Copy Product Info
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Ethyl glucuronide는 에탄올의 비휘발성, 수용성 직접 대사산물로 체액, 조직 및 모발에서 검출될 수 있다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 17685-04-0
Sample solution is provided at 25 µL, 10mM.
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Ethyl glucuronide는 에탄올의 비휘발성, 수용성 직접 대사산물로 체액, 조직 및 모발에서 검출될 수 있다. Ethyl glucuronide 함량은 알코올 섭취량과 양의 상관관계가 있고 알코올 남용 검출을 위한 마커로 사용될 수 있다[2]. Ethyl glucuronide는 관련 화합물의 동정을 위한 액체 크로마토그래피-탠덤 질량 분석법 개발을 위한 표준 내부 대조 그룹으로 사용될 수 있다[3].
체외 실험에서 100μM 농도의 Ethyl glucuronide 처리 24시간은 TLR4-MD2 복합체의 형성을 차단하고 HEK-TLR4 세포에서 TLR4 의존적 SEAP 단백질의 발현을 자극하였다[4].
체내 실험에서 0.38µg 단일 용량의 척수강 내 투여를 통한 Ethyl glucuronide 처리는 3시간 이내에 쥐에서 현저한 통각 과민증을 유발하였다[4].
References:
[1] Wurst F M, Kempter C, Metzger J, et al. Ethyl glucuronide: a marker of recent alcohol consumption with clinical and forensic implications[J]. Alcohol, 2000, 20(2): 111-116.
[2] Helander A, Böttcher M, Fehr C, et al. Detection times for urinary ethyl glucuronide and ethyl sulfate in heavy drinkers during alcohol detoxification[J]. Alcohol & Alcoholism, 2009, 44(1): 55-61.
[3] Wurst F M, Kempter C, Seidl S, et al. Ethyl glucuronide--a marker of alcohol consumption and a relapse marker with clinical and forensic implications[J]. Alcohol and alcoholism (Oxford, Oxfordshire), 1999, 34(1): 71-77.
[4] Lewis S S, Hutchinson M R, Zhang Y, et al. Glucuronic acid and the ethanol metabolite ethyl-glucuronide cause toll-like receptor 4 activation and enhanced pain[J]. Brain, behavior, and immunity, 2013, 30: 24-32.
| 세포 실험 [1]: | |
세포 라인 | HEK-TLR4 세포 |
제조 방법 | HEK-TLR4 세포는 DMEM 배지에 10% 태아 소혈청 (FBS)과 항생제 (페니실린 1000U/ml, 스트렙토마이신 10mg/ml)를 보충해서 정상 조건(5% CO₂, 95% 가습 공기) 하에서 배양하였습니다. HEK-TLR4 세포 (5×10³개/웰)를 96웰 세포 배양판에 접종하고 37°C, 5% CO₂ 조건에서 48시간 배양하였습니다. 이 후에 세포를 각기 다른 농도의 Ethyl glucuronide (0.01, 0.1, 1, 10, 100µM)로 48시간 처리하고 각 웰에서 상청액(15μl)을 수집해서 SEAP 수준을 측정하였습니다. |
반응 조건 | 0.01, 0.1, 1, 10, 100µM; 24 시간 동안 |
응용 분야 | Ethyl glucuronide 처리는 용량 의존적으로 HEK-TLR4 세포 내 SEAP 수준을 증가시켰습니다. |
References: | |
| Cas No. | 17685-04-0 | SDF | |
| Canonical SMILES | OC([C@@H]1-;@[C@H](-;@[C@@H](-;@[C@@H](O)-;@[C@H](OCC)-;@O-;@1)O)O)=O | ||
| Formula | C8H14O7 | M.Wt | 222.19 |
| Solubility | H2O : 125 mg/mL (562.58 mM; Need ultrasonic) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.5007 mL | 22.5033 mL | 45.0065 mL |
| 5 mM | 900.1 μL | 4.5007 mL | 9.0013 mL |
| 10 mM | 450.1 μL | 2.2503 mL | 4.5007 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)