LDAO (Synonyms: Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide) |
Catalog No.GC44046
One-Click Copy Product Info
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LDAO(Lauryl Dimethylamine Oxide)는 막 단백질을 용해하고 안정시키기에 사용할 수 있는 비이온/양음이온 세제이다.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1643-20-5
Sample solution is provided at 25 µL, 10mM.
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LDAO(Lauryl Dimethylamine Oxide)는 막 단백질을 용해하고 안정시키기에 사용할 수 있는 비이온/양음이온 세제이다. LDAO의 온화한 세정 능력은 단백질의 원래 구조를 유지하면서 지질 이중층 구조를 효과적으로 파괴할 수 있다[2]. LDAO는 종종 세균이나 포유류의 세포막에서 경막 단백질 (G 단백질 결합 수용체 등)을 용해 하면서 기능적 활성을 유지하는데 자주 사용된다[3]. LDAO는 미토콘드리아막 구조를 용해함으로써 H⁺-ATPase의 활성을 간접적으로 영향을 미쳐서 ATP 수해리 과정을 조절할 수 있다[4]. LDAO를 구리 없는 클릭 화학 반응에 추가하면 CRM197 변이체의 연쇄상구균의 결합 효율이 현저하게 증가한다[5]. Rhodovulum sulfidophilum의 성장 매질에 LDAO를 추가하면 카로티노이드 생합성이 급격한 변화가 나타났다[6].
References:
[1] Veldhuis G, Gabellieri E, Poolman B, et al. The effect of the detergent-shell on the structure of a membrane-protein[J]. Mechanics of the mannitol transporter from Escherichia coli, 111.
[2] Orwick‐Rydmark M, Arnold T, Linke D. The use of detergents to purify membrane proteins[J]. Current protocols in protein science, 2016, 84(1): 4.8. 1-4.8. 35.
[3] Marcovich I. Structural studies of enzymes: a bacterial flippase and a human kinase[J]. 2020.
[4] Vazquez-Laslop N, Dreyfus G. Mitochondrial H+-ATPase activation by an amine oxide detergent[J]. Journal of Biological Chemistry, 1986, 261(17): 7807-7810.
[5] Bautista L, Pill-Pepe L, Kapoor N, et al. Addition of lauryldimethylamine N-oxide (LDAO) to a copper-free click chemistry reaction improves the conjugation efficiency of a cell-free generated CRM197 variant to clinically important Streptococcus pneumoniae serotypes[J]. ACS omega, 2022, 7(39): 34921-34928.
[6] Hagemann G E, Sturgis J N, Wagner E, et al. The effects of the detergent LDAO on the carotenoid metabolism and growth of Rhodovulum sulfidophilum[J]. Microbiological research, 1996, 151(4): 421-426.
| Cas No. | 1643-20-5 | SDF | |
| Synonyms | Dimethyldodecylamine N-oxide, Lauramine Oxide, Lauryldimethylamine Oxide, N-Lauryldimethylamine N-oxide | ||
| Canonical SMILES | CCCCCCCCCCCC[N+](C)([O-])C | ||
| Formula | C14H31NO | M.Wt | 229.4 |
| Solubility | DMF: 0.3 mg/ml,DMSO: 0.1 mg/ml,Ethanol: 15 mg/ml,PBS (pH 7.2): 0.1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.3592 mL | 21.796 mL | 43.592 mL |
| 5 mM | 871.8 μL | 4.3592 mL | 8.7184 mL |
| 10 mM | 435.9 μL | 2.1796 mL | 4.3592 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)