SB-4 |
Catalog No.GC50593
One-Click Copy Product Info
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SB-4 is a potent benzoxazole Bone morphogenetic protein 4 (BMP4) signaling agonist with an EC50 value of 73.64nM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 100874-08-6
Sample solution is provided at 25 µL, 10mM.
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SB-4 is a potent benzoxazole Bone morphogenetic protein 4 (BMP4) signaling agonist with an EC50 value of 73.64nM[1]. SB-4 activates the BMP4 signaling pathway, leading to an increase in the phosphorylation levels of key second messengers (SMAD-1/5/9), thereby activating BMP target genes such as ID1 and ID3[2]. SB-4 has been widely used to promote the differentiation of bone marrow mesenchymal stem cells (BMSCs) into glial cells[3].
In vitro, SB-4 treatment for 48h significantly inhibited viability of AtT20/D16v-F2 cells, with an IC50 value of 39.05μM[4]. Treatment with 200μM SB-4 for 4 days induced the expression levels of Blimp1, Prdm14, Nnose3 and Stella in the epiblast-like cells (EpiLCs)[5]. Treatment with 10μM SB-4 for 21 days promoted osteogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs), and increased the levels of alkaline phosphatase and osteopontin[6].
In vivo, SB-4 treatment via intraperitoneal injection at a dose of 5mg/kg every 2 days for 12 days mitigated the placental senescence phenotype in Grem1-overexpressing pregnant mice, alleviated placental dysfunction, and reduced the expression of classical senescence markers[7]. A single intraperitoneal injection of SB-4 (5mg/kg) increased the survival rate of mice exposed to lethal radiation (8Gy) during 21 days and promoted the recovery of blood cells[8].
References:
[1] Bradford S T J, Ranghini E J, Grimley E, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds[J]. Journal of Biological Chemistry, 2019, 294(9): 3125-3136.
[2] Lin Z B, Zou P, Ma X Y, et al. Metabolic stress-activated endothelial STING rewires the sinusoidal immune niche in MASLD[J]. The Journal of Clinical Investigation, 2026.
[3] Changmeng Z, Hongfei W, Cheung M C H, et al. Revealing the developmental origin and lineage predilection of neural progenitors within human bone marrow via single-cell analysis: implications for regenerative medicine[J]. Genome Medicine, 2023, 15(1): 66.
[4] Xu L, Ning R, Du X, et al. Bone morphogenetic protein signaling agonist SB4 (BMPSB4) inhibits corticotroph pituitary neuroendocrine tumors by activation of autophagy via a BMP4/SMADs-dependent pathway[J]. ACS Pharmacology & Translational Science, 2024, 7(7): 1951.
[5] Tehrani R S H, Sayahpour F A, Esfandiari F. A comparison between BMP4 and SB4 in inducing germ line gene expression pattern during embryonic stem cells differentiation[J]. Differentiation, 2022, 123: 9-17.
[6] Naghshnejad F, Zeynali B, Shabani I, et al. Effects of Bone Morphogenetic Protein Agonist Small Molecule, SB4, on Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells[J]. Journal of Bone Metabolism, 2026, 33(1): 18.
[7] Tian H, Wei Y, Zhang L, et al. Aberrant expression of Gremlin1 induces trophoblast senescence in the placenta of advanced maternal age pregnancy[J]. Journal of Translational Medicine, 2026.
[8] Li Y, Li Y, Zhang B, et al. Single-cell transcriptomics reveals BMP4-BMPR2 signaling promotes radiation resistance in hematopoietic stem cells following injury[J]. Nature Communications, 2025, 16(1): 5470.
| Cell experiment [1]: | |
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Cell lines |
AtT20/D16v-F2 cells |
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Preparation Method |
AtT20/D16v-F2 cells were cultivated in DMEM medium supplemented with 100IU/ml penicillin, and 100μg/ml streptomycin and 10% fetal bovine serum at 37°C in an incubator with 5% CO2. Cells (1×104 cells /well) were seeded in the 96-well plates and cultured overnight followed by treatment with different concentrations of SB-4 (0, 1, 5, 10, and 100μM) for 48h. Cells viability was measured. |
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Reaction Conditions |
0, 1, 5, 10, and 100μM; 48h |
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Applications |
SB-4 reduced cell viability of AtT20/D16v-F2 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6-Ly5.2 (CD45.2) mice |
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Preparation Method |
C57BL/6-Ly5.2 (CD45.2) mice were housed in a specific pathogen-free (SPF) barrier environment and were continuously provided with sterilized food, water, and bedding. For survival experiments, mice were intraperitoneally injected with a single dose of SB-4 (5mg/kg), or vehicle, 2 hours before exposure to 8Gy irradiation using a 60Co irradiator. After irradiation, the mice were immediately returned to a SPF barrier environment. Daily observations and recordings were made of the mice’s survival status during 30 days. |
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Dosage form |
5mg/kg; once; i.p. |
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Applications |
SB-4 treatment increased the survival rate of mice exposed to lethal radiation (8Gy) during 30 days. |
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References: [1] Xu L, Ning R, Du X, et al. Bone morphogenetic protein signaling agonist SB4 (BMPSB4) inhibits corticotroph pituitary neuroendocrine tumors by activation of autophagy via a BMP4/SMADs-dependent pathway[J]. ACS Pharmacology & Translational Science, 2024, 7(7): 1951. [2] Li Y, Li Y, Zhang B, et al. Single-cell transcriptomics reveals BMP4-BMPR2 signaling promotes radiation resistance in hematopoietic stem cells following injury[J]. Nature Communications, 2025, 16(1): 5470. |
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| Cas No. | 100874-08-6 | SDF | |
| Canonical SMILES | BrC1=CC=C(C=C1)CSC2=NC3=C(C=CC=C3)O2 | ||
| Formula | C14H10BrNOS | M.Wt | 320.2 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.123 mL | 15.6152 mL | 31.2305 mL |
| 5 mM | 624.6 μL | 3.123 mL | 6.2461 mL |
| 10 mM | 312.3 μL | 1.5615 mL | 3.123 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)