Guanosine-5'-triphosphate disodium salt (Synonyms: GTP) |
Catalog No.GC61932
One-Click Copy Product Info
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Guanosine-5'-triphosphate disodium salt is a nucleotide that plays a critical role in biosynthesis and cellular signal transduction.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 56001-37-7
Sample solution is provided at 25 µL, 10mM.
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Guanosine-5'-triphosphate disodium salt is a nucleotide that plays a critical role in biosynthesis and cellular signal transduction[1]. Guanosine-5'-triphosphate disodium salt serves as a phosphate group donor, participates in protein synthesis processes, and activates G protein-mediated signaling pathways[2]. Guanosine-5'-triphosphate disodium salt acts as a high-energy mononucleotide precursor in the enzymatic biosynthesis of DNA and RNA. Guanosine-5'-triphosphate disodium salt can promoting the differentiation of myogenic cells and showing potential for applications in studies such as skeletal muscle regeneration[3-4].
In vitro, treatment of PC12 cells with Guanosine-5'-triphosphate disodium salt (300μM) for 48 hours promotes neurite outgrowth[5]. Treatment of C2C12 mouse myoblasts with Guanosine-5'-triphosphate disodium salt (500μM) for 48 hours induces a transient increase in intracellular calcium concentration, activates intermediate-conductance calcium-activated potassium channels (IKCa), and leads to membrane hyperpolarization[6]. Exposure of human myogenic precursor cells to Guanosine-5'-triphosphate disodium salt (500μM) for 24 hours significantly upregulates the expression of the myogenic regulator Myogenin and specifically enhances the expression of miR-133a and miR-133b[7]. Treatment of PC12 cells with Guanosine-5'-triphosphate disodium salt (300μM) for 48 hours induces a sustained increase in intracellular calcium concentration by activating specific GTP-binding sites on the cell surface (Kd=52.9μM), an effect dependent on the activation of L-type voltage-gated calcium channels[8].
References:
[1] Pendergrass JC, Haley BE. Inhibition of brain tubulin-guanosine 5'-triphosphate interactions by mercury: similarity to observations in Alzheimer's diseased brain. Met Ions Biol Syst. 1997;34:461-78.
[2] Kaziro Y. The role of guanosine 5'-triphosphate in polypeptide chain elongation. Biochim Biophys Acta. 1978 Sep 21;505(1):95-127.
[3] Lin D, Cui F, Bu Q, et al. The expression and clinical significance of GTP-binding RAS-like 3 (ARHI) and microRNA 221 and 222 in prostate cancer. J Int Med Res. 2011;39(5):1870-5.
[4] Pietrangelo T, Guarnieri S, Fulle S, et al. Signal transduction events induced by extracellular guanosine 5' triphosphate in excitable cells. Purinergic Signal. 2006 Nov;2(4):633-6.
[5] Gysbers JW, Rathbone MP. GTP and guanosine synergistically enhance NGF-induced neurite outgrowth from PC12 cells. Int J Dev Neurosci. 1996 Feb;14(1):19-34.
[6] Pietrangelo T, Fioretti B, Mancinelli R, et al. Extracellular guanosine-5'-triphosphate modulates myogenesis via intermediate Ca(2+)-activated K+ currents in C2C12 mouse cells. J Physiol. 2006 May 1;572(Pt 3):721-33.
[7] Pietrangelo T, Di Filippo ES, Locatelli M, et al. Extracellular Guanosine 5'-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine. Front Pharmacol. 2018 Mar 16;9:152.
[8] Gysbers JW, Guarnieri S, Mariggiò MA, et al. Extracellular guanosine 5' triphosphate enhances nerve growth factor-induced neurite outgrowth via increases in intracellular calcium. Neuroscience. 2000;96(4):817-24.
| Cell experiment [1]: | |
Cell lines | PC12 cells (rat pheochromocytoma cell line) |
Preparation Method | PC12 cells were maintained in RPMI-1640 medium supplemented with 5% fetal calf serum and 5% heat-inactivated horse serum. For neurite outgrowth assays, cells were plated on poly-DL-ornithine-coated plates in medium containing 1.5% serum and treated with Guanosine-5'-triphosphate disodium salt (300µM) in the presence or absence of 40ng/ml Nerve Growth Factor (NGF). |
Reaction Conditions | 300µM; 48h. |
Applications | Extracellular Guanosine-5'-triphosphate disodium salt synergistically enhanced NGF-induced neurite outgrowth, increasing the proportion of cells with neurites from 20-35% (NGF alone) to 40-65%. This effect was mediated by a sustained increase in intracellular Ca²⁺ concentration ([Ca²⁺]i) via influx through L-type voltage-gated calcium channels and Ca²⁺-induced Ca²⁺ release (CICR) from intracellular ryanodine-sensitive stores. |
References: | |
| Cas No. | 56001-37-7 | SDF | |
| Synonyms | GTP | ||
| Canonical SMILES | O[C@@H]([C@H]([C@H](N1C=NC2=C1N=C(N)NC2=O)O3)O)[C@H]3COP([O-])(OP(O)(OP([O-])(O)=O)=O)=O.[Na+].[Na+] | ||
| Formula | C10H14N5Na2O14P3 | M.Wt | 567.14 |
| Solubility | Water : 125 mg/mL (220.40 mM) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7632 mL | 8.8162 mL | 17.6323 mL |
| 5 mM | 352.6 μL | 1.7632 mL | 3.5265 mL |
| 10 mM | 176.3 μL | 881.6 μL | 1.7632 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)