Bz-VGR-AMC TFA is a fluorescent substrate for the trypsin-like activity of the 20S proteasome. Bz-VGR-AMCTFA can be used to measure the trypsin-like (β2) activity of the 20S proteasome (Ex/Em = 360/460 nm) [1] [2].
In Vitro, Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs). 1. Take the tissue sample to be tested, dilute it with ddH2O, and mix it with the reaction buffer (250 mM HEPES, pH 7.5, 5 mM EDTA, 0.5% NP-40, 0.01% SDS, 5 mM ATP). 2. Add the fluorescent peptide Bz-VGR-AMC (10 μM) TFA to the sample. 3. Incubate the reaction at 37°C for 30 minutes. 4. Monitor the fluorescence on a fluorescence microplate reader at a wavelength of Ex/Em = 360/460 nm.
References:
[1]. Zhong YL, et al. Celastrol induce apoptosis of human multiple myeloma cells involving inhibition of proteasome activity. Eur J Pharmacol. 2019 Jun 15;853:184-192.
[2]. Jarome TJ, et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci. 2013 Aug 30;7:115.
In Vitro, Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs). 1. Take the tissue sample to be tested, dilute it with ddH2O, and mix it with the reaction buffer (250 mM HEPES, pH 7.5, 5 mM EDTA, 0.5% NP-40, 0.01% SDS, 5 mM ATP). 2. Add the fluorescent peptide Bz-VGR-AMC (10 μM) TFA to the sample. 3. Incubate the reaction at 37°C for 30 minutes. 4. Monitor the fluorescence on a fluorescence microplate reader at a wavelength of Ex/Em = 360/460 nm.
References:
[1]. Zhong YL, et al. Celastrol induce apoptosis of human multiple myeloma cells involving inhibition of proteasome activity. Eur J Pharmacol. 2019 Jun 15;853:184-192.
[2]. Jarome TJ, et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci. 2013 Aug 30;7:115.