Emeramide (BDTH2) (Synonyms: BDTH2, NBMI) |
Catalog No.GC31671
One-Click Copy Product Info
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Emeramide (BDTH2) es un quelante y antioxidante de metales pesados solubles en lípidos
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 351994-94-0
Sample solution is provided at 25 µL, 10mM.
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Emeramide (BDTH2) es un quelante y antioxidante de metales pesados solubles en lípidos [1]. Emeramide forma fuertes complejos de tiosulfuro covalentes o semicovalentes con “metales blandos” (como el mercurio (Hg2+), el plomo (Pb2+), y el cadmio (Cd2+)), reduciendo así la concentración libre y la toxidad de estos metales [2]. Emeramide puede ayudar a restaurar o proteger los sistemas intracelulares de tiol/antioxidantes, como el glutatión (GSH), aliviando así el estrés oxidativo inducido por metales [3]. Emeramide es principalmente un agente terapéutico potencial para el envenenamiento de metales pesados[4].
En las células U-87 MG, Emeramide (0-200µM; 24h) reduce la viabilidad celular a altas concentraciones [5]. En las células HepG2, el pretratamiento con Emeramide (0-100µM; 24h) redujo la muerte celular inducida por CuSO4 y disminuyó los niveles de ROS [6].
En ratones mutantes knock-in H67D, Emeramide oral (450mg/kg; po; 6 semanas) reduce la acumulación de hierro en el cerebro y el hígado [7].
References:
[1]. Secor J D, Kotha S R, Gurney T O, et al. Novel lipid-soluble thiol-redox antioxidant and heavy metal chelator, N, N′-bis (2-mercaptoethyl) isophthalamide (NBMI) and phospholipase D-specific inhibitor, 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) attenuate mercury-induced lipid signaling leading to protection against cytotoxicity in aortic endothelial cells[J]. International journal of toxicology, 2011, 30(6): 619-638.
[2]. Wang Y, Zhang S, Bian J, et al. A method for the rapid detection of heavy metal mercury ions based on a novel mercury chelator N, N'-bis (2-mercaptoethyl) isophthalamide[J]. Food Chemistry, 2025, 468: 142486.
[3]. Secor J D, Kotha S R, Gurney T O, et al. Novel lipid-soluble thiol-redox antioxidant and heavy metal chelator, N, N′-bis (2-mercaptoethyl) isophthalamide (NBMI) and phospholipase D-specific inhibitor, 5-fluoro-2-indolyl des-chlorohalopemide (FIPI) attenuate mercury-induced lipid signaling leading to protection against cytotoxicity in aortic endothelial cells[J]. International journal of toxicology, 2011, 30(6): 619-638.
[4]. Geier D A, Geier M R. Reductions in plasma and urine mercury concentrations following N, N′ bis-(2-mercaptoethyl) isophthalamide (NBMI) therapy: a post hoc analysis of data from a randomized human clinical trial[J]. Biometals, 2024, 37(2): 433-445.
[5]. Gadde R, Betharia S. N, N′ bis-(2-mercaptoethyl) isophthalamide (NBMI) exerts neuroprotection against lead-induced toxicity in U-87 MG cells[J]. Archives of Toxicology, 2021, 95(8): 2643-2657.
[6]. Gadde R, Betharia S. NBMI Exerts Protection Against Copper Overload in HepG2 and SH‐SY5Y Cell Lines[J]. The FASEB Journal, 2022, 36.
[7]. Cheng R, Gadde R, Fan Y, et al. Reversal of genetic brain iron accumulation by N, N′-bis (2-mercaptoethyl) isophthalamide, a lipophilic metal chelator, in mice[J]. Archives of Toxicology, 2022, 96(7): 1951-1962.
| Experimentos celulares [1]: | |
Líneas celulares | Células U-87 MG |
Método de preparación | Las células se chaparon en placas opacas de fondo transparente de 96 pozos a una densidad de 2 × 104 celdas/pocillo. Después de la unión durante la noche, fueron tratados con PbAc (0-300µM) durante 24 o 48h, Emeramide (0-200µM) durante 24h o DMSA (0-500µM) durante 24h para establecer perfiles de toxicidad individuales. |
Condiciones de reacción | 0-200 µM; 24h |
Áreas de aplicación | Emeramide reduce la viabilidad celular a altas concentraciones. |
| Experimentos con animales [2]: | |
Modelos animales | Ratones mutantes de golpe H67D |
Método de preparación | Los ratones mutantes knock-in H67D y los ratones de tipo salvaje (WT) de control (5-6 semanas de edad, n = 6-8 por grupo) se mantuvieron en un ciclo de luz/oscuridad de 12 horas, y se les dio comida y agua ad libitum. A los ratones se les administró Emeramide (450 mg/kg de peso corporal), deferiprona (100 mg/kg) o vehículo (0,5 % de carboximetilcelulosa) por vía corporal oral diariamente durante 6 semanas. |
Forma de dosificación | 450 mg/kg; po; 6 semanas |
Áreas de aplicación | Emeramide oral reduce la acumulación de hierro en el cerebro y el hígado. |
References: | |
| Cas No. | 351994-94-0 | SDF | |
| Sinónimos | BDTH2, NBMI | ||
| Canonical SMILES | O=C(C1=CC=CC(C(NCCS)=O)=C1)NCCS | ||
| Formula | C12H16N2O2S2 | M.Wt | 284.4 |
| Solubility | DMSO : 100 mg/mL (351.62 mM);Water : < 0.1 mg/mL (insoluble) | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5162 mL | 17.5809 mL | 35.1617 mL |
| 5 mM | 703.2 μL | 3.5162 mL | 7.0323 mL |
| 10 mM | 351.6 μL | 1.7581 mL | 3.5162 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)