2'-Thiouridine is a key modified nucleoside at the wobble position of tRNA anticodons. 2'-Thiouridine mainly exists in tRNAs for glutamic acid, glutamine, lysine, etc., and participates in precise decoding regulation [1].
In Vitro, 2'-Thiouridine ensures the specificity of codon-anticodon recognition by stabilizing the base-pairing conformation and restricting abnormal wobble pairing, thereby improving translation fidelity and efficiency[1].
References:
[1]. Agris PF, et al. Biological function of 2-thiouridine in Escherichia coli glutamic acid transfer ribonucleic acid. Biochemistry. 1973 Oct 1;12(22):4331-7.
In Vitro, 2'-Thiouridine ensures the specificity of codon-anticodon recognition by stabilizing the base-pairing conformation and restricting abnormal wobble pairing, thereby improving translation fidelity and efficiency[1].
References:
[1]. Agris PF, et al. Biological function of 2-thiouridine in Escherichia coli glutamic acid transfer ribonucleic acid. Biochemistry. 1973 Oct 1;12(22):4331-7.