Phleomycin (Synonyms: Zeocin) |
Catalog No.GC14081
One-Click Copy Product Info
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Phleomycin es un antibiótico glicopéptido y un inductor de rotura de hebras de DNA (DNA-SB) que se puede utilizar para seleccionar células genéticamente diseñadas.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11006-33-0
Sample solution is provided at 25 µL, 10mM.
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Phleomycin es un antibiótico glicopéptido y un inductor de rotura de hebras de DNA (DNA-SB) que se puede utilizar para seleccionar células genéticamente diseñadas[1]. Phleomycin es una molécula que se encuentra en Streptomyces, y similar a la bleomicina, puede unirse e insertarse en el DNA, interrumpiendo la integridad de la estructura de la doble hélice[2]. Phleomycin tiene un efecto mutagénico en las cepas resistentes de Streptococcus[3].
In vitro, el tratamiento de células HeLa con Phleomycin (100-400μg/mL) durante 0-24h inhibió la proliferación celular de una manera dependiente del tiempo y la dosis, indujo la citotoxicidad, indujo la liberación inicial de citocromo c, la regulación a la baja de Bcl-XL, ENDOG, DAXX y MDM2, y la regulación positiva de CASP yd BID[4]. El tratamiento de las células de Nannochloropsis oceanica con Phleomycin (0.2μg/mL) durante 48 horas causó roturas aleatorias de doble cadena en el DNA de las células algas y aumentó la eficiencia de transformación del gen de resistencia activa a la hygromycin B (aph7)[5]. El tratamiento con Phleomycin (0-200ng/mL) de embryonic fibroblasts de ratón (MEFs) de diferentes mutantes durante 48 horas redujo significativamente la tasa de supervivencia de los MEFs que carecen de XRCC4, DNA-Ligase IV o Xlf, pero no tuvo un efecto significativo en los MEFs de mutantes PAXX y MEFs de tipo salvaje[6].
References:
[1] Ma X, Pan K, Zhang L, et al. Genetic transformation of Nannochloropsis oculata with a bacterial phleomycin resistance gene as dominant selective marker[J]. Journal of Ocean University of China, 2016, 15: 351-356.
[2] Stokowa-Sołtys K, Dzyhovskyi V, Wieczorek R, et al. Phleomycin complex–Coordination mode and in vitro cleavage of DNA[J]. Journal of inorganic biochemistry, 2019, 195: 71-82.
[3] Van Peer A F, De Bekker C, Vinck A, et al. Phleomycin increases transformation efficiency and promotes single integrations in Schizophyllum commune[J]. Applied and Environmental Microbiology, 2009, 75(5): 1243-1247.
[4] Hwang J, Kim Y Y, Huh S, et al. The Time‐Dependent Serial Gene Response to Zeocin Treatment Involves Caspase‐Dependent Apoptosis in HeLa Cells[J]. Microbiology and immunology, 2005, 49(4): 331-342.
[5] Zhang Z, Guo L, Liu H, et al. Zeocin treatment significantly elevated transformation efficiency of Nannochloropsis oceanica[J]. Journal of Applied Phycology, 2022, 34(3): 1587-1594.
[6] Abramowski V, Etienne O, Elsaid R, et al. PAXX and Xlf interplay revealed by impaired CNS development and immunodeficiency of double KO mice[J]. Cell Death & Differentiation, 2018, 25(2): 444-452.
| Experimentos celulares [1]: | |
Líneas celulares | Células HeLa |
Método de preparación | Se permitió que las células (6×104 por pocillo) se unieran en placas de 24 pocillos durante 1 día antes de agregar Phleomycin y los cultivos se midieron a 0, 6 , 12, 18, 24h y 100, 200, 300, 400μg/mL de Phleomycin con un hemacytometer. Los efectos citotóxicos de la Phleomycin en las células HeLa se determinaron mediante un recuento de células viables y un ensayo MTT. |
Condiciones de reacción | 100, 200, 300, 400μg/mL; 0-24h |
Áreas de aplicación | Phleomycin inhibe la proliferación de células HeLa e induce la citotoxicidad de una manera dependiente de la dosis y del tiempo. |
References: | |
| Cas No. | 11006-33-0 | SDF | |
| Sinónimos | Zeocin | ||
| Chemical Name | (2R,3S,4S,5R,6R)-2-(((2R,3S,4S,5S,6S)-2-(2-(6-amino-2-(3-amino-1-((2,3-diamino-3-oxopropyl)amino)-3-oxopropyl)-5-methylpyrimidine-4-carboxamido)-3-((5-((1-((2-(4-((4-guanidinobutyl)carbamoyl)-4',5'-dihydro-[2,4'-bithiazol]-2'-yl)ethyl)amino)-3-hydroxy-1-o | ||
| Canonical SMILES | NC(CNC(CC(N)=O)C1=NC(N)=C(C(C(NC(C(NC(C(C(C)C(NC(C(NCCC2=NC(C3=NC(C(NCCCCNC(N)=N)=O)=CS3)CS2)=O)C(O)C)=O)O)C)=O)C(O[C@H]4[C@]([H])([C@H]([C@@H]([C@@H](O4)CO)O)O)O[C@H]5O[C@@H]([C@H]([C@@H]([C@@H]5O)OC(N)=O)O)CO)C6=CNC=N6)=O)=N1)C)C(N)=O.[Cu+2].Cl | ||
| Formula | C55H85N20O21S2Cu • HCl | M.Wt | 1526.5 |
| Solubility | ≤10mg/ml in PBS, pH 7.2 | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 655.1 μL | 3.2755 mL | 6.5509 mL |
| 5 mM | 131 μL | 655.1 μL | 1.3102 mL |
| 10 mM | 65.5 μL | 327.5 μL | 655.1 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)