TPPU
Catalog No.: GC15387Purity:>98.00%
TPPU is a soluble epoxide hydrolase (sEH) inhibitor with IC50 values of 37 and 3.7 nM for monkey and human sEH.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1222780-33-7
10mM (in 1mL DMSO)In Stock
$49.00
1
1mgIn Stock
$25.00
1
5mgIn Stock
$45.00
1
10mgIn Stock
$73.00
1
25mgIn Stock
$140.00
1
50mgIn Stock
$224.00
1
100mgIn Stock
$335.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $49.00 | In Stock | 1 | |
| 1mg | $25.00 | In Stock | 1 | |
| 5mg | $45.00 | In Stock | 1 | |
| 10mg | $73.00 | In Stock | 1 | |
| 25mg | $140.00 | In Stock | 1 | |
| 50mg | $224.00 | In Stock | 1 | |
| 100mg | $335.00 | In Stock | 1 |
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com
Citations
2 publicationsPublications from researchers using this productDescription
TPPU is a soluble epoxide hydrolase (sEH) inhibitor with IC50 values of 37 and 3.7 nM for monkey and human sEH. TPPU has anti-inflammatory, analgesic, antiatherogenic and antihypertensive effects[1-2].
TPPU(1–10 μM; 48 h) reversed CORT-induced cell death in PC12 cells[3]. TPPU(0.1–10 μM; 24 h) protects tau from H2O2-induced hyperphosphorylation in HEK293/tau cells by regulating PI3K/AKT/GSK-3β pathway[4].
TPPU (i.p.; 10 mg/kg) treatment decreases serum dihydroxyeicosatrienoic acid (DHET) levels and serum IL-6 levels following burn injury, preventing DHET induced neutrophil depression in burn injury mice model [5]. TPPU(0-5 mg/L TPPU; delivered in drinking water; 1 week) increased exercise-induced effects on inhibiting cardiac enlargement and improving cardiac function by increasing EET levels in MI mice[6]. Pre-treatment with TPPU(0.1 mg/kg/d; i.g) attenuated seizures severity during status epilepticus (SE) and depressive behaviours during the period of epileptogenesis[7].
References:
[1]. Ulu A, Appt S, et,al. Pharmacokinetics and in vivo potency of soluble epoxide hydrolase inhibitors in cynomolgus monkeys. Br J Pharmacol. 2012 Mar;165(5):1401-12. doi: 10.1111/j.1476-5381.2011.01641.x. PMID: 21880036; PMCID: PMC3372725.
[2]. Li J, Wen Z, et,al. Soluble epoxide hydrolase inhibitor promotes the healing of oral ulcers. Clinics (Sao Paulo). 2023 May 4;78:100208. doi: 10.1016/j.clinsp.2023.100208. PMID: 37148830; PMCID: PMC10192938.
[3]. Wu Q, Song J, et,al. TPPU, a sEH Inhibitor, Attenuates Corticosterone-Induced PC12 Cell Injury by Modulation of BDNF-TrkB Pathway. J Mol Neurosci. 2019 Mar;67(3):364-372. doi: 10.1007/s12031-018-1230-z. Epub 2019 Jan 14. PMID: 30644034.
[4]. Yao ES, Tang Y, et,al. TPPU protects tau from H2O2-induced hyperphosphorylation in HEK293/tau cells by regulating PI3K/AKT/GSK-3β pathway. J Huazhong Univ Sci Technolog Med Sci. 2016 Dec;36(6):785-790. doi: 10.1007/s11596-016-1662-z. Epub 2016 Dec 7. PMID: 27924507.
[5]. Bergmann CB, Hammock BD, et,al. TPPU treatment of burned mice dampens inflammation and generation of bioactive DHET which impairs neutrophil function. Sci Rep. 2021 Aug 16;11(1):16555. doi: 10.1038/s41598-021-96014-2. PMID: 34400718; PMCID: PMC8368302.
[6]. Guo Y, Luo F, et,al. TPPU enhanced exercise-induced epoxyeicosatrienoic acid concentrations to exert cardioprotection in mice after myocardial infarction. J Cell Mol Med. 2018 Mar;22(3):1489-1500. doi: 10.1111/jcmm.13412. Epub 2017 Dec 19. PMID: 29265525; PMCID: PMC5824362.
[7]. Peng W, Shen Y et,al. TPPU Pre-Treatment Rescues Dendritic Spine Loss and Alleviates Depressive Behaviours during the Latent Period in the Lithium Chloride-Pilocarpine-Induced Status Epilepticus Rat Model. Brain Sci. 2021 Nov 5;11(11):1465. doi: 10.3390/brainsci11111465. PMID: 34827464; PMCID: PMC8615907.
Protocol
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Cell experiment [1]: |
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Cell lines |
PC12 cells |
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Preparation method |
PC12 cells were exposed to 200 μM CORT for 48 h and then with 1-10 μM TPPU. |
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Reaction Conditions |
1-10 μM; 48 h |
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Applications |
TPPU reversed CORT-induced cell death in PC12 cells. |
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Animal experiment [2]: |
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Animal models |
C57BL/6 mice |
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Preparation method |
Myocardial infarction(MI) mouse model was established. After 1 week recovery, the surviving mice were randomly divided into four groups: non-myocardial infarction and non-exercise training (NMI NET), non-myocardial infarction with exercise training (NMI ET), myocardial infarction and non-exercise training (MI NET), and myocardial infarction with exercise training (MI ET).Mice in the ET groups underwent 4 weeks of exercise. To test the effect of TPPU on exercised mice, mice in the MI ET group were fed 0, 0.2, 1 or 5 mg/L TPPU for 1 week prior to the MI surgery and subsequent 4-week exercise programme. |
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Dosage form |
0-5 mg/L TPPU; delivered in drinking water; 1 week |
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Applications |
TPPU increased exercise-induced effects on inhibiting cardiac enlargement and improving cardiac function by increasing epoxyeicosatrienoic acid (EET) levels in MI mice. |
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References: [1]. Wu Q, Song J,et,al. TPPU, a sEH Inhibitor, Attenuates Corticosterone-Induced PC12 Cell Injury by Modulation of BDNF-TrkB Pathway. J Mol Neurosci. 2019 Mar;67(3):364-372. doi: 10.1007/s12031-018-1230-z. Epub 2019 Jan 14. PMID: 30644034. [2]. Guo Y, Luo F et,al. TPPU enhanced exercise-induced epoxyeicosatrienoic acid concentrations to exert cardioprotection in mice after myocardial infarction. J Cell Mol Med. 2018 Mar;22(3):1489-1500. doi: 10.1111/jcmm.13412. Epub 2017 Dec 19. PMID: 29265525; PMCID: PMC5824362. |
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Product Documents
Purity:>98.00%Appearance:A solid
Chemical Properties
CAS No.
1222780-33-7
Chemical Name
N-[1-(1-oxopropyl)-4-piperidinyl]-N’-[4-(trifluoromethoxy)phenyl)-urea
SMILES
CCC(N(CC1)CCC1NC(NC2=CC=C(OC(F)(F)F)C=C2)=O)=O
Molecular Formula
C16H20F3N3O3
Molecular Weight
359.3
Solubility
≤5mg/ml in ethanol;12.5mg/ml in DMSO;15mg/ml in dimethyl formamide
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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