Windorphen |
Catalog No.GC12133
One-Click Copy Product Info
|
Wnt inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 19881-70-0
Sample solution is provided at 25 µL, 10mM.
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Windorphen is a Wnt inhibitor that selectively abrogates the Wnt signaling for ventral development.
Wnt protein is a family of signaling proteins that interacts on the Wnt/β-Catenin pathways during embryotic development, cell proliferation, migration and adult tissue homeostasis.
In canonical Wnt/β-catenin reporter cell line STF293, Windorphen dose-dependently inhibits Wnt3a-inducible TOPFLASH-luciferase activity (IC50 of 1.5 mM). In human colon adenocarcinoma
SW480 cells with defective APC gene that constitutively activates Wnt signaling, 72 h treatment of Windorphen causes a wide spread apoptosis. [1]
In zebrafish embryos, Windorphen leads to apparent expansion of the dorsal markers (pax2.1) and marker of rhombomeres 3 and 5 at the six-somite stage (krox0). Windorphen selectively inhibits Wnt signaling in ventral and lateral regions of the 50% epiboly stage embryo (5.3 hpf). In addition, Windoprhen treatment rescues the telencephalon/eye phenotype in the mbl mutant zebrafish (i.e. a defective Axin1/β-catenin destruction complex causes abnormal activation of Wnt signaling and the loss of telencephalon and eyes.) [1]
Reference:
1. Hao J, Ao A, Zhou L et al. Selective small molecule targeting β-catenin function discovered by in
vivo chemical genetic screen. Cell Rep. 2013 Sep 12;4(5):898-904.
| Cas No. | 19881-70-0 | SDF | |
| Chemical Name | (E)-3-chloro-2,3-bis(4-methoxyphenyl)acrylaldehyde | ||
| Canonical SMILES | O=C/C(C1=CC=C(OC)C=C1)=C(Cl)\C2=CC=C(OC)C=C2 | ||
| Formula | C17H15ClO3 | M.Wt | 302.75 |
| Solubility | ≥ 11.45mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3031 mL | 16.5153 mL | 33.0306 mL |
| 5 mM | 660.6 μL | 3.3031 mL | 6.6061 mL |
| 10 mM | 330.3 μL | 1.6515 mL | 3.3031 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)