APF is a novel ROS indicator that selectively and dose-dependently fluoresces upon binding to certain reactive oxygen species in solution and in cells with a maximum excitation/emission wavelength of 490/515 nm. APF has higher specificity and stability than HDCFDA. APF is cell membrane permeable, non-fluorescent, and reacts with reactive oxygen species, such as hydroxyl radical, peroxynitroso anion, or hypochlorite anion, to produce strong green fluorescence, which can be detected by fluorescence microscopy or flow cytometry. After reacting with hydroxyl radicals, peroxynitrite anion or hypochlorite anion, APF produces strong green fluorescence, which can be detected by fluorescence microscope, high throughput imager, fluorescence zymography or flow cytometry. At the same time, it can also be coupled with a variety of molecules to achieve specific labeling detection, which is widely used in the fields of biomarkers, cell imaging and fluorescence microscopy[1].
References:
[1]. Cohn C A, Pedigo C E, Hylton S N, et al. Evaluating the use of 3'-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions[J]. Geochemical Transactions, 2009, 10: 1-9.