GLPBio

Formononetin

Catalog No.: GN10527Purity:>99.50%Synonyms: Biochanin B, NSC 93360
COASDSData Sheet
Formononetin is a potent FGFR2 inhibitor with an IC50 of 4.3μM. Formononetin potently inhibits angiogenesis and tumor growth.

المنتجات مخصصة للاستخدام البحثي فقط، وليست للاستخدام البشري. نحن لا نبيع للمرضى.

Formononetin
Cas No.: 485-72-3
10mM (in 1mL DMSO)In Stock
$35.00
1
10mgIn Stock
$32.00
1
50mgIn Stock
$63.00
1
100mgIn Stock
$84.00
1
200mgIn Stock
$116.00
1
500mgIn Stock
$182.00
1
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المراجع (Citations)

لا تتوفر حاليًا مراجع لهذا المنتج. فيما يلي مجموعة مختارة من المنشورات التي تستشهد بمنتجات GlpBio.
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    187(9):2288-2304 (2024)
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    183(7):1867-1883 (2020)
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الوصف (Description)

Formononetin is a potent FGFR2 inhibitor with an IC50 of 4.3μM. Formononetin potently inhibits angiogenesis and tumor growth[1].

Formononetin (10, 80μM; 3d) inhibit Ishikawa, HEC-1A, and HEC-251 cells proliferation in a dose-dependent manner[2]. Formononetin (10, 25μM; 24h) is a potent protectant against cisplatin-induced cell death through inhibiting intracellular ROS accumulation in pig kidney epithelial LLC-PK1 cells[3]. Formononetin (0.1, 0.3, 1μM; 30-60min) increased CNE2 cell estrogen receptor-α (ERα) and bcl-2, but decreased protein-phosphatase and tensin homologue (PTEN) protein expression[4].

In mice hyperoxic acute lung injury, Formononetin (10, 100 mg/kg; ip; 72h) significantly attenuated hyperoxia-induced elevated lung water content, up-regulation of pro-inflammatory cytokine levels, and increased lung neutrophil infiltration[5]. In mice acute ischaemic injury model, formononetin (20mg/kg; po; 28d) promoted the expression of ER β and p53 in a dose-dependent manner[6].

References:
[1] Wu X Y, Xu H, Wu Z F, et al. Formononetin, a novel FGFR2 inhibitor, potently inhibits angiogenesis and tumor growth in preclinical models[J]. Oncotarget, 2015, 6(42): 44563.
[2]. Zhang Q, Huang X. The modulatory properties of Astragalus membranaceus treatment on endometrial cancer: an integrated pharmacological method[J]. PeerJ, 2021, 9: e11995.
[3] Lee H, Lee D, Kang K S, et al. Inhibition of intracellular ROS accumulation by Formononetin attenuates cisplatin-mediated apoptosis in LLC-PK1 cells[J]. International journal of molecular sciences, 2018, 19(3): 813.
[4] Guo Y H, Tang F Y, Wang Y, et al. Low concentration of Formononetin promotes proliferation of estrogen receptor-positive cells through an ERα-miR-375-PTEN-ERK1/2-bcl-2 pathway[J]. Oncotarget, 2017, 8(59): 100045.
[5]Chen Y, Wei D, Zhao J, et al. Reduction of hyperoxic acute lung injury in mice by Formononetin[J]. PloS one, 2021, 16(1): e0245050.
[6] Zhao L, Han J, Liu J, et al. A novel Formononetin derivative promotes anti-ischemic effects on acute ischemic injury in mice[J]. Frontiers in Microbiology, 2021, 12: 786464.

البروتوكول (Protocol)

Cell experiment [1]:

Cell lines

The Ishikawa, HEC-1A, and HEC-251 cell lines

Preparation Method

The effect of Formononetin on the colony-forming ability of endometrial cancer cells was evaluated by colony formation assay. The Ishikawa, HEC-1A, and HEC-251 cell lines were seeded into 6-well plates at the concentration of 400 cells per well. After incubating for 24h at 37°C, the cells were incubated with Formononetin (40µM and 80µM). Three days later, most single colonies contain more than 50 cells. Cells were harvested and rinsed three times with distilled water, and images were obtained by a microscope.

Reaction Conditions

10, 80μM; 3d

Applications

Formononetin inhibit Ishikawa, HEC-1A, and HEC-251 cells proliferation in a dose-dependent manner.
Animal experiment [2]:

Animal models

Hyperoxic acute lung injury Modle

Preparation Method

Before the experiment, mice were housed in closed and individually ventilated cages under a 12h light:dark cycle for at least 1 week to acclimate to the environment. Mice had ad libitum access to standard chow and water. Mice were divided into a control group (room air+vehicle) and four other groups (room air+Formononetin, hyperoxia+vehicle, hyperoxia+Formononetin, and zinc protoporphyrin (ZnPP, an inhibitor of HO-1)+hyperoxia+Formononetin). To induce hyperoxic acute lung injury, mice were housed in closed cages and exposed to hyperoxia (>95% oxygen) for 72h. Room air exposure served as a control.

Dosage form

10, 100mg/kg; ip; 72h

Applications

Formononetin pretreatment notably attenuated hyperoxia-induced elevating pulmonary water content, upregulation of proinflammatory cytokine levels and increasing infiltration of neutrophil in the lung.

References:
[1]. Zhang Q, Huang X. The modulatory properties of Astragalus membranaceus treatment on endometrial cancer: an integrated pharmacological method[J]. PeerJ, 2021, 9: e11995.
[2]. Chen Y, Wei D, Zhao J, et al. Reduction of hyperoxic acute lung injury in mice by Formononetin[J]. PloS one, 2021, 16(1): e0245050.

وثائق المنتج (Product Documents)

Purity:>99.50%Appearance:A solid
COASDSData Sheet

الخصائص الكيميائية (Chemical Properties)

رقم CAS
485-72-3
المرادفات
Biochanin B, NSC 93360
الاسم الكيميائي
7-hydroxy-3-(4-methoxyphenyl)chromen-4-one
SMILES
COC1=CC=C(C=C1)C2=COC3=C(C2=O)C=CC(=C3)O
الصيغة الجزيئية
C16H12O4
الوزن الجزيئي
268.27
الذوبانية
DMF: 30 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 25 mg/ml
التخزين
Store at -20°C
إرشادات عامة
اختر مذيبًا مناسبًا وفقًا لبيانات ذوبانية المنتج. بعد التحضير، قسّم المحلول المخزون إلى أجزاء لتجنب دورات التجميد والذوبان المتكررة.
استخدم المحاليل المخزونة خلال 6 أشهر عند حفظها في -80°C، أو خلال شهر واحد عند حفظها في -20°C.
لتحسين الذوبانية، سخّن الأنبوب إلى 37°C وعرّضه للموجات فوق الصوتية لمدة مناسبة.
شروط الشحن
تُشحن عينات التقييم مع عبوات تبريد. وتُشحن الأحجام الأخرى المتاحة في درجة حرارة الغرفة، أو مع عبوات تبريد عند الطلب.

الحاسبات (Calculators)المولارية / التخفيف / الوزن الجزيئي / تحويل الوحدات / تركيبة داخل الجسم الحي / الذوبانية

g/mol

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