Ginsenoside Rb1 (Synonyms: Gypenoside III, NSC 310103) |
رقم الكتالوجGN10156
One-Click Copy Product Info
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Ginsenoside Rb1 is a natural active ingredient extracted from ginseng that can modulate the activity of multiple signaling pathways.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 41753-43-9
Sample solution is provided at 25 µL, 10mM.
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Ginsenoside Rb1 is a natural active ingredient extracted from ginseng that can modulate the activity of multiple signaling pathways. Ginsenoside Rb1 inhibits Na⁺,K⁺-ATPase activity (IC₅₀=6.3±1.0μM). Ginsenoside Rb1 can be used in research on diseases such as cancer, cardiovascular and cerebrovascular diseases, and asthma[1-4].
In vitro, WI-38 cells were treated with Ginsenoside Rb1 (20–30μM) and LPS (2.5–10mg/ml) for 24h. Ginsenoside Rb1 significantly reversed the LPS-induced decrease in cell viability, apoptosis, and production of pro-inflammatory factors (IL-1β, IL-6, TNF-α); Ginsenoside Rb1 downregulated miR-222 expression and inhibited NF-κB pathway activation[5]. HUVECs were pretreated with Ginsenoside Rb1 (10–20μM) for 30min, followed by stimulation with H₂O₂ (60μM) for 1h and then cultured for another 24h. Ginsenoside Rb1 significantly reduced the number of SA-β-gal-positive cells and PAI-1 expression, while increasing eNOS expression and NO production, and restored SIRT1 expression and AMPK phosphorylation[6].
In vivo, aged C57BL/6J mice received intraperitoneal injections of Ginsenoside Rb1 (20mg/kg; 6 days per week) for 6 weeks. Ginsenoside Rb1 significantly alleviated age-related aortic structural remodeling and DNA damage[7]. In ovariectomized C57BL/6J mice, Ginsenoside Rb1 (12.5 or 50mg/kg; single or 7 days) was administered intraperitoneally 1 hour before ISO (200mg/kg) treatment. Ginsenoside Rb1 significantly attenuated ISO-induced myocardial injury and cardiomyocyte necrosis[8].
References:
[1] Cao J, Zheng YQ, Liu TP, et al. Inhibitory effects of ginsenoside Rg1 and Rb1 on rat brain microsomal Na+,K(+)-ATPase activity. Zhongguo Yao Li Xue Bao. 1990 Jan;11(1):10-4.
[2] Zhang J, Cao L, Wang H, et al. Ginsenosides Regulate PXR/NF-κB Signaling and Attenuate Dextran Sulfate Sodium-Induced Colitis. Drug Metab Dispos. 2015 Aug;43(8):1181-9.
[3] Joh EH, Lee IA, Jung IH, et al. Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation--the key step of inflammation. Biochem Pharmacol. 2011 Aug 1;82(3):278-86.
[4] He S, Ye H, Wang Q, et al. Ginsenoside Rb1 targets to HO-1 to improve sepsis by inhibiting ferroptosis. Free Radic Biol Med. 2025 Jan;226:13-28.
[5] Wei E, Fang X, Jia P, et al. Ginsenoside Rb1 Alleviates Lipopolysaccharide-Induced Inflammatory Injury by Downregulating miR-222 in WI-38 Cells. Cell Transplant. 2021 Jan-Dec;30:9636897211002787.
[6] Zheng Z, Wang M, Cheng C, et al. Ginsenoside Rb1 reduces H2O2‑induced HUVEC dysfunction by stimulating the sirtuin‑1/AMP‑activated protein kinase pathway. Mol Med Rep. 2020 Jul;22(1):247-256.
[7] Si Q, Wu L, Zhang X, et al. Ginsenoside Rb1 attenuates arterial aging by reducing DNA damage in aged mice. Exp Gerontol. 2026 Feb;214:113011.
[8] Wang Y, Tang X, Cui J, et al. Ginsenoside Rb1 mitigates acute catecholamine surge-induced myocardial injuries in part by suppressing STING-mediated macrophage activation. Biomed Pharmacother. 2024 Jun;175:116794.
| Cell experiment [1]: | |
Cell lines | HUVECs (human umbilical vein endothelial cells) |
Preparation Method | HUVECs were maintained in M199 medium supplemented with 20% fetal bovine serum and 60μg/ml endothelial cell growth supplement at 37°C in a 5% CO₂ incubator. HUVECs were pretreated with Ginsenoside Rb1 at concentrations of 10 or 20μM for 30min prior to H₂O₂ treatment. |
Reaction Conditions | 10-20μM; pretreatment 30min. |
Applications | Ginsenoside Rb1 significantly decreased the SA-β-gal-positive cell number and attenuated cell senescence induced by H₂O₂ in a dose-dependent manner. Ginsenoside Rb1 restored the H₂O₂-induced reduction of SIRT1 expression and activation of AMPK phosphorylation. Ginsenoside Rb1 increased endothelial nitric oxide synthase expression and nitric oxide production, and suppressed PAI-1 expression. |
| Animal experiment [2]: | |
Animal models | C57BL/6JGpt mice (72-week-old) |
Preparation Method | Mice were randomly grouped and received intraperitoneal injections of either PBS or 20mg/kg Ginsenoside Rb1 for 6 weeks (6 days of injections followed by 1 day of rest per week). Young mice (12-week-old) served as controls. Aortic tissues were analyzed using histomorphology, atomic force microscopy, senescence biomarkers, apoptosis assay, and DNA damage detection. |
Dosage form | 20mg/kg; i.p.; 6 days per week for 6 weeks. |
Applications | Ginsenoside Rb1 significantly alleviated structural remodeling of the aging aorta, restored smooth muscle cell alignment and extracellular matrix organization. Ginsenoside Rb1 ameliorated aging-induced impairments in nanobiomechanical properties. Ginsenoside Rb1 downregulated p21 expression, reduced SA-β-gal positive area percentage, decreased apoptotic cells, and significantly alleviated the elevation of γ-H2AX in the aging aorta. |
References: | |
| Cas No. | 41753-43-9 | SDF | |
| المرادفات | Gypenoside III, NSC 310103 | ||
| Chemical Name | Ginsenoside Rb1 | ||
| Canonical SMILES | CC(=CCCC(C)(C1CCC2(C1C(CC3C2(CCC4C3(CCC(C4(C)C)OC5C(C(C(C(O5)CO)O)O)OC6C(C(C(C(O6)CO)O)O)O)C)C)O)C)OC7C(C(C(C(O7)COC8C(C(C(C(O8)CO)O)O)O)O)O)O)C | ||
| Formula | C54H92O23 | M.Wt | 1109.29 |
| الذوبان | ≥ 51.1mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 901.5 μL | 4.5074 mL | 9.0148 mL |
| 5 mM | 180.3 μL | 901.5 μL | 1.803 mL |
| 10 mM | 90.1 μL | 450.7 μL | 901.5 μL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
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- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)