Cholera toxin (choleragen)
Catalog No.: GC31655Purity:>95.00%Synonyms: Choleragen，CT
Cholera toxin (choleragen) is an enterotoxogenic protein complex consisting of a single A subunit (cholera toxin A subunit (CTA)) and a pentamer of B subunits (cholera toxin B subunit (CTB)).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9012-63-9
500μgIn Stock
$555.00
1
1mgIn Stock
$841.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 500μg | $555.00 | In Stock | 1 | |
| 1mg | $841.00 | In Stock | 1 |
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com
Citations
3 publicationsPublications from researchers using this productDescription
Cholera toxin (choleragen) is an enterotoxogenic protein complex consisting of a single A subunit (cholera toxin A subunit (CTA)) and a pentamer of B subunits (cholera toxin B subunit (CTB)). It enters the host cell by binding to ganglioside GM1 on the plasma membrane and retrograde into the endoplasmic reticulum via the trans-Golgi network[1-3].
Cholera toxin(0.5ug/ml;3h) treatment of Jurkat cells for 3 h caused a nearly total disappearence of the TcR complex from teh surface of the cell[4,5]. Cholera toxin enters host intestinal epithelia cells, and its retrograde transport to the cytosol results in the massive loss of fluids and electrolytes associated with severe dehydration[6]. Cholera toxin activates adenylate cyclase by catalyzing ADP-ribosylation of Gs alpha, the stimulatory guanine nucleotide-binding protein[7].
References:
[1]. van Heyningen S. Cholera toxin. Biol Rev Camb Philos Soc. 1977 Nov;52(4):509. doi: 10.1111/j.1469-185x.1977.tb00858.x. PMID: 203347.
[2]. Wernick NL, Chinnapen DJ, et,al. Cholera toxin: an intracellular journey into the cytosol by way of the endoplasmic reticulum. Toxins (Basel). 2010 Mar;2(3):310-25. doi: 10.3390/toxins2030310. Epub 2010 Mar 5. PMID: 22069586; PMCID: PMC3153193.
[3]. Kellner A, Taylor M, et,al. A binding motif for Hsp90 in the A chains of ADP-ribosylating toxins that move from the endoplasmic reticulum to the cytosol. Cell Microbiol. 2019 Oct;21(10):e13074. doi: 10.1111/cmi.13074. Epub 2019 Jul 5. PMID: 31231933; PMCID: PMC6744307.
[4]. Sommermeyer H, Schwinzer R,et,al. Cholera toxin modulates the T cell antigen receptor/CD3 complex but not the CD2 molecule and inhibits signaling via both receptor structures in the human T cell lymphoma Jurkat. Eur J Immunol. 1989 Dec;19(12):2387-90. doi: 10.1002/eji.1830191232. PMID: 2575034.
[5]. Nunes J, Bagnasco M, et,al. Cholera toxin inhibits the increase in cytoplasmic free calcium induced via the CD2 pathway of human T-lymphocyte activation. J Cell Biochem. 1989 Apr;39(4):391-400. doi: 10.1002/jcb.240390405. PMID: 2542344.
[6]. Wands AM, Cervin J, et,al. Fucosylated Molecules Competitively Interfere with Cholera Toxin Binding to Host Cells. ACS Infect Dis. 2018 May 11;4(5):758-770. doi: 10.1021/acsinfecdis.7b00085. Epub 2018 Feb 22. PMID: 29411974; PMCID: PMC5948155.
[7]. Tsai SC, Noda M, et,al. Stimulation of choleragen enzymatic activities by GTP and two soluble proteins purified from bovine brain. J Biol Chem. 1988 Feb 5;263(4):1768-72. PMID: 3123477.
Protocol
| Cell experiment [1]: | |
|
Cell lines |
Jurkat cells |
|
Preparation Method |
Cells were incubated for 3 h with medium alone or with Cholera toxin (0.5 ug/ml) in medium. After washing the cells the expression of the indicated antigen on the cell surface was measured by FCM analysis. |
|
Reaction Conditions |
0.5ug/ml;3h |
|
Applications |
Cholera toxin treatment of Jurkat cells for 3 h caused a nearly total disappearence of the TcR complex from teh surface of the cell. |
|
References: [1]. Nunes J, Bagnasco M, et,al. Cholera toxin inhibits the increase in cytoplasmic free calcium induced via the CD2 pathway of human T-lymphocyte activation. J Cell Biochem. 1989 Apr;39(4):391-400. doi: 10.1002/jcb.240390405. PMID: 2542344. |
|
Product Documents
Purity:>95.00%Appearance:A solid
Chemical Properties
CAS No.
9012-63-9
Synonyms
Choleragen，CT
SMILES
[Cholera toxin]
Solubility
When reconstituted to 0.5 ml with water, the buffer is 0.05 M Tris, 0.2 M NaCl, 0.001 M Na2EDTA at pH 7.5
Storage
Store at 4°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion
g/mol
Reviews
Review for Cholera toxin (choleragen)
Rating: 5
- 5 Star100%
- 4 Star0%
- 3 Star0%
- 2 Star0%
- 1 Star0%