ChX710 |
Catalog No.GC34329
One-Click Copy Product Info
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ChX710 is a small-molecule compound that primes the type I interferon response to cytosolic DNA.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2438721-44-7
Sample solution is provided at 25 µL, 10mM.
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ChX710 is a small-molecule compound that primes the type I interferon response to cytosolic DNA. ChX710 induces the interferon-stimulated response element (ISRE) promoter, specific interferon-stimulated genes (ISGs), and phosphorylation of interferon regulatory factor (IRF)3, effectively priming the cellular response to DNA transfection via the STING pathway[1].
In vitro, ChX710 (6–50μM) was applied to cell lines such as HEK-293 for 8–48 hours. ChX710 specifically activates the ISRE promoter and induces phosphorylation of IRF3. ChX710 effectively enhances the cellular type I interferon response to cytosolic (transfected) DNA, leading to increased IFN-β secretion. At higher concentrations (25–50µM), ChX710 exhibits significant cytotoxicity, reducing cellular ATP levels and viability[1].
References:
[1] Khiar S, Lucas-Hourani M, Nisole S, et al. Identification of a small molecule that primes the type I interferon response to cytosolic DNA. Sci Rep. 2017 May 31;7(1):2561.
| Cell experiment [1]: | |
Cell lines | HEK-293T (human embryonic kidney epithelial cells), A549 (human lung epithelial adenocarcinoma cells), MRC5 (human lung fibroblasts), Vero (African green monkey kidney epithelial cells), and primary human peripheral blood mononuclear cells (PBMCs) |
Preparation Method | Reporter cell lines (e.g., STING-37, a HEK-293 cell line stably expressing luciferase under the control of ISRE) or other cell types were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin at 37°C and 5% CO₂. Cells were treated with ChX710 (6-50µM) or related compounds for specified durations. For synergy experiments, cells were co-treated with ChX710 and transfected with plasmid DNA or stimulated with 2',3'-cGAMP using JetPrime transfection reagent. |
Reaction Conditions | 6-50µM; 8-48 hours. |
Applications | ChX710 specifically activates the Interferon-Stimulated Response Element (ISRE) promoter. This activation is dependent on the adaptor protein MAVS and the transcription factor IRF1, but independent of STAT1/STAT2. ChX710 induces phosphorylation of IRF3 but is insufficient to trigger type I interferon (IFN-α/β) secretion on its own. ChX710 induces a modest, cell-type-specific profile of Interferon-Stimulated Genes (ISGs). Crucially, ChX710 potently primes and synergistically enhances the cellular type I interferon response to cytosolic (transfected) DNA, leading to a strong, STING-dependent increase in IFN-β secretion and ISG expression. ChX710 exhibits significant cytotoxicity at higher concentrations, reducing cellular ATP levels and viability. |
References: | |
| Cas No. | 2438721-44-7 | SDF | |
| Canonical SMILES | O=C(NC(C)CN(C)C)C1=C(N=C(C2=CC=NC=C2)N3)C3=CC=C1 | ||
| Formula | C18H21N5O | M.Wt | 323.39 |
| Solubility | DMSO : 20 mg/mL (61.84 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0922 mL | 15.4612 mL | 30.9224 mL |
| 5 mM | 618.4 μL | 3.0922 mL | 6.1845 mL |
| 10 mM | 309.2 μL | 1.5461 mL | 3.0922 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 23 reference(s) in Google Scholar.)