Chktide acetate is a synthetic peptide substrate applicable for activity assays of Chk1 kinase and cyk/CaMKII. Chktide acetate contains the Ser53 phosphorylation site of Xenopus cyclin B2 [1] [2].
In Vitro, Chktide acetate serves as a standard synthetic substrate for Chk1 serine protein kinase assays and can be used for the quantitative detection of Chk1 catalytic activity[1]. Chktide acetate contains the Ser53 phosphorylation site of Xenopus cyclin B2, and upon phosphorylation, it induces a prophase-like premature phenotype in fibroblasts[2].
References:
[1]. Walker M, et al. Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity. Oncogene. 2009 Jun 18;28(24):2314-23.
[2]. Stevens I, et al. Identification of cyk, a cyclin B2 kinase, as a novel calcium/calmodulin-dependent protein kinase II and its role during Xenopus laevis oocyte maturation. Experimental cell research. 1999 Nov 01;252(2):303-18.
In Vitro, Chktide acetate serves as a standard synthetic substrate for Chk1 serine protein kinase assays and can be used for the quantitative detection of Chk1 catalytic activity[1]. Chktide acetate contains the Ser53 phosphorylation site of Xenopus cyclin B2, and upon phosphorylation, it induces a prophase-like premature phenotype in fibroblasts[2].
References:
[1]. Walker M, et al. Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity. Oncogene. 2009 Jun 18;28(24):2314-23.
[2]. Stevens I, et al. Identification of cyk, a cyclin B2 kinase, as a novel calcium/calmodulin-dependent protein kinase II and its role during Xenopus laevis oocyte maturation. Experimental cell research. 1999 Nov 01;252(2):303-18.