EGNHS |
Katalog-Nr.GC61794
One-Click Copy Product Info
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EGNHS ist ein Alkyl/Ether-basierter PROTAC-Linker, der bei der Synthese von PROTACs verwendet werden kann.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 70539-42-3
Sample solution is provided at 25 µL, 10mM.
EGNHS is a proteolytic targeting chimera (PROTAC) linker, which belongs to the alkyl/ether class and can be used to synthesize PROTAC molecules[1]. PROTAC is a hybrid bifunctional small molecule compound, a "three-body" polymer - target protein ligand-linker-E3 ligand[2]. EGNHS can form a cross-link between Lys232 and Lys241 of horseradish peroxidase C (HRP) and modify Lys174 without forming a cross-link[3]. EGNHS is a cross-linker containing a reactive NHS ester (N-hydroxysuccinimide) end. NHS ester is a commonly used primary amine reactive group. It can react with primary amine groups to form a stable amide bond, which helps to effectively label the target molecule[4]. EGNHS chemically modified porcine trypsin to obtain EG-trypsin, which improved the thermal stability and catalytic performance of trypsin[5].
References:
[1] An S, Fu L. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs[J]. EBioMedicine, 2018, 36: 553-562.
[2] Li R, Liu M, Yang Z, et al. Proteolysis-targeting chimeras (PROTACs) in cancer therapy: present and future[J]. Molecules, 2022, 27(24): 8828.
[3] O'Brien A M, Ó'Fágáin C, Nielsen P F, et al. Location of crosslinks in chemically stabilized horseradish peroxidase: implications for design of crosslinks[J]. Biotechnology and bioengineering, 2001, 76(4): 277-284.
[4] Flynne W G. Biotechnology and bioengineering[M]. Nova Publishers, 2008.
[5] Colleary S, O'fagain C. Stability and catalytic properties of chemically modified pig trypsin[J]. Biocatalysis and Biotransformation, 2009, 27(5-6): 309-317.
| Cas No. | 70539-42-3 | SDF | |
| Canonical SMILES | O=C(OCCOC(CCC(ON1C(CCC1=O)=O)=O)=O)CCC(ON2C(CCC2=O)=O)=O | ||
| Formula | C18H20N2O12 | M.Wt | 456.36 |
| Löslichkeit | DMSO: 100 mg/mL (219.13 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1913 mL | 10.9563 mL | 21.9125 mL |
| 5 mM | 438.3 μL | 2.1913 mL | 4.3825 mL |
| 10 mM | 219.1 μL | 1.0956 mL | 2.1913 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 36 reference(s) in Google Scholar.)