HBC (HBC 530) (Synonyms: HBC 530) |
Katalog-Nr.GC19622
One-Click Copy Product Info
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HBC ist ein grÜn fluoreszierendes Protein (GFP) Fluorophor-Ähnlicher synthetischer Farbstoff mit einem strukturell starren Elektronenakzeptor und einem starken Elektronendonor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 156840-13-0
Sample solution is provided at 25 µL, 10mM.
HBC (HBC 530) is a GFP-like synthetic dye with the maximum excitation wavelength of 485nm, and the maximum emission wavelength of 530nm [1]. HBC features a structurally rigid electron acceptor and a strong electron donor, which can bind to RNA of different lengths and emit intense fluorescence[2]. The unique fluorescence imaging channel provided by HBC has been widely used in the engineering of luminometric sensor platforms for multiple colors RNA labeling or intracellular RNA-RNA interaction imaging [3]. HBC can be used for in situ localization of tumor-specific proteases and animal optical in in vitro imaging analysis experiments [4].
References:
[1] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] Bereiter R, Micura R. Synthesis of HBC fluorophores with an electrophilic handle for covalent attachment to Pepper RNA[J]. Beilstein Journal of Organic Chemistry, 2025, 21(1): 727-735.
[3] Tang H, Peng J, Peng S, et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. Chemical Science, 2022, 13(47): 14032-14040.
[4] Xie X, Sun Y, Peng J, et al. Collagen anchoring protein-nucleic acid chimeric probe for in situ in vivo mapping of a tumor-specific protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
HBC530-based analysis of metalloproteinase 2 (MMP-2)
This protocol only provides a guideline, and should be modified according to your specific needs.
1. Preparation of HBC530 solution
2μM HBC530 working solution was prepared from 1mM HBC530 stock solution (dissolved in DMSO). NOTE: The working solution must be prepared fresh on the day of the experiment.
2. Imaging of MMP-2 by HBC530 in the extracellular matrix (ECM)
(1) For imaging of MMP-2 in the ECM, the HePG2 cells were pretreated with TGF-β for 24h.
(2) The 10μl of 100μM protein-nucleic acid chimeric probe (PNCP) was first prepared in the reaction buffer (50mM HEPES, 50mM NaCl, 10μM MnCl2, and 1mM MgCl2) at 37°C for 6h, and then 100μl of the transcription buffer containing 1μM PNCP and 4mM rNTP was added to the cell culture dish for 2h.
(3) 2μM HBC530 working solution was incubated with HepG2 for 15min. A microplate reader was used to record the fluorescence signals.
3. Caveats.
(1) Dark operation: HBC530 working solution has a half-life of 4h at 4°C, and is prepared and used on demand.
(2) During the entire experiment, make sure to wear protective gloves for proper safety precautions.
(3) The reagents used in this experiment are for scientific research purposes only.
References:
[1] Xie X, Sun Y, Peng J, et al. Collagen anchoring protein-nucleic acid chimeric probe for in situ in vivo mapping of a tumor-specific protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
| Cas No. | 156840-13-0 | SDF | |
| Überlieferungen | HBC 530 | ||
| Canonical SMILES | OCCN(C)C1=CC=C(/C=C(C2=CC=C(C#N)C=C2)\C#N)C=C1 | ||
| Formula | C19H17N3O | M.Wt | 303.36 |
| Löslichkeit | insoluble in EtOH; insoluble in Water; >21.2 mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.2964 mL | 16.4821 mL | 32.9641 mL |
| 5 mM | 659.3 μL | 3.2964 mL | 6.5928 mL |
| 10 mM | 329.6 μL | 1.6482 mL | 3.2964 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)