GLPBio

Trofinetide (NNZ-2566)

Catalog No.: GC30804Purity:>98.00%Synonyms: NNZ-2566
COASDSData Sheet
Trofinetide (NNZ-2566) (NNZ-2566), ein synthetisches Analogon des endogenen N-Terminus-Tripeptids Glycin-Prolin-Glutamat (GPE), hat sich in Tiermodellen fÜr Hirnverletzungen als neuroprotektiv erwiesen.

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Trofinetide (NNZ-2566)
Cas No.: 853400-76-7
1mgIn Stock
$39.00
1
5mgIn Stock
$70.00
1
10mgIn Stock
$119.00
1
25mgIn Stock
$237.00
1
50mgIn Stock
$368.00
1
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Zitate (Citations)

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    Nature
    641, 529–536 (2025)
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    628, 630–638 (2024)
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    Nature
    632, 686–694 (2024)
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    Nature
    618, 1017–1023 (2023)
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    Nature
    610, 366–372 (2022)
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    Cell
    187(9):2288-2304 (2024)
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    Cell
    183(7):1867-1883 (2020)
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    Science
    388(6745) (2025)
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    387(6739) (2025)
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    387(6734) (2025)
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    35, 97–116 (2025)
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    34, 683–706 (2024)
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    33, 904–922 (2023)
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    31, 1291–1307 (2021)

Beschreibung (Description)

Trofinetide (NNZ-2566) is a synthetic analogue of the endogenous N-terminal tripeptide Glycine Proline-Glutamate (GPE) with neuroprotective effects [1]. Trofinetide can cross the blood-brain barrier and improve synaptic function, repair synaptic structure, reduce neuroinflammatory substances in the brain, and enhance antioxidant response[2]. Trofinetide has been widely used in Fmr1 knockout mice to correct learning and memory deficits, abnormal hyperactivity, and social interaction impairments [3].

In vitro, Trofinetide pretreatment at 1μM for 2 hours effectively reversed the cytotoxicity of Aβ42 (10µM; 24h) on BV2 cells and increased cell viability[4]. Treatment with 10μM Trofinetide for 24h reversed okadaic acid-induced cell death in isolated embryonic striatal neurons[5].

In vivo, Trofinetide treatment via continuous intravenous infusion at a rate of 3mg/kg/h for 12 hours inhibited neuroinflammation and proinflammatory cytokine expression induced by experimental penetrating ballistic-like brain injury in rats[6]. Intravenous infusion of Trofinetide (10mg/kg/h) for 4 hours significantly reduced infarct size in rats caused by middle cerebral artery occlusion (MCAO) and attenuated weight loss[7].

References:
[1] Neul J L, Percy A K, Benke T A, et al. Trofinetide for the treatment of Rett syndrome: a randomized phase 3 study[J]. Nature Medicine, 2023, 29(6): 1468-1475.
[2] Hudu S A, Elmigdadi F, Qtaitat A A, et al. Trofinetide for Rett syndrome: highlights on the development and related inventions of the first USFDA-approved treatment for rare pediatric unmet medical need[J]. Journal of Clinical Medicine, 2023, 12(15): 5114.
[3] Deacon R M J, Glass L, Snape M, et al. NNZ-2566, a novel analog of (1–3) IGF-1, as a potential therapeutic agent for fragile X syndrome[J]. Neuromolecular medicine, 2015, 17(1): 71-82.
[4] Chen M, Ning Y, Yang H, et al. Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis[J]. Molecular Neurobiology, 2026, 63(1): 129.
[5] Bickerdike M J, Thomas G B, Batchelor D C, et al. NNZ-2566: A Gly–Pro–Glu analogue with neuroprotective efficacy in a rat model of acute focal stroke[J]. Journal of the neurological sciences, 2009, 278(1-2): 85-90.
[6] Wei H H, Lu X C M, Shear D A, et al. NNZ-2566 treatment inhibits neuroinflammation and pro-inflammatory cytokine expression induced by experimental penetrating ballistic-like brain injury in rats[J]. Journal of neuroinflammation, 2009, 6(1): 19.
[7] Bickerdike M J, Thomas G B, Batchelor D C, et al. NNZ-2566: A Gly–Pro–Glu analogue with neuroprotective efficacy in a rat model of acute focal stroke[J]. Journal of the neurological sciences, 2009, 278(1-2): 85-90.

Protokoll (Protocol)

Cell experiment [1]:

Cell lines

BV2 cells

Preparation Method

BV2 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) at 37°C in 5% CO2. Cells were plated onto a 96-well plate at a density of 3×103 cells/ml for 24h, and the cells were incubated with different concentrations of Trofinetide 1µM for 2h, followed by co-incubation with Aβ42 oligomers (10µM) for an additional 24h. After 72 hours, cell viability was analyzed.

Reaction Conditions

1µM; 2h

Applications

Trofinetide pretreatment significantly rescued Aβ42-induced cytotoxicity in BV2 cells.
Animal experiment [2]:

Animal models

Male Sprague-Dawley rats

Preparation Method

Male Sprague-Dawley rats were weighed and anaesthetized with 5% halothane/O2 and maintained with 3% halothane/O2 throughout surgery. A blunt 22G×8.5mm guide cannula was stereotaxically implanted and rested on the dura, positioned to enable an injection cannula to be lowered adjacent to the middle cerebral artery (0.2mm anterior, 5.2mm lateral to Bregma) and was anchored in place with dental cement. To enable intravenous (i.v.) infusion of Trofinetide, rats were surgically fitted with an indwelling catheter into the jugular vein and housed individually in metabolic cages before induction of the stroke-like injury. Three to four days following surgery, a stroke-like injury was induced. A 15.8mm 30G needle connected to a 1ml syringe with polythene tubing was inserted into the guide cannula, and ET-1 (100pmol in 3µl) was infused over a period of approximately 3min to induce vasoconstriction of the middle cerebral artery. Following stroke injury, Trofinetide or vehicle was administered intravenously via the indwelling catheter (10mg/kg/h for 4h, starting 3h after stroke). Five days following drug treatment, rats were sacrificed, and the brains were taken for histological analysis.

Dosage form

30mg/kg/h for 4h; i.v.

Applications

Trofinetide treatment reduced brain injury size in rats subjected to focal stroke.

References:
[1] Chen M, Ning Y, Yang H, et al. Trofinetide Improves Cognitive Function in APP/PS1 Mice by Suppressing Inflammation and Apoptosis[J]. Molecular Neurobiology, 2026, 63(1): 129.
[2] Bickerdike M J, Thomas G B, Batchelor D C, et al. NNZ-2566: A Gly–Pro–Glu analogue with neuroprotective efficacy in a rat model of acute focal stroke[J]. Journal of the neurological sciences, 2009, 278(1-2): 85-90.

Produktdokumente (Product Documents)

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemische Eigenschaften (Chemical Properties)

CAS-Nr.
853400-76-7
Synonyme
NNZ-2566
SMILES
O=C(O)CC[C@@H](C(O)=O)NC([C@@]1(C)N(C(CN)=O)CCC1)=O
Summenformel
C13H21N3O6
Molekulargewicht
315.32
Löslichkeit
Water : ≥ 50 mg/mL (158.57 mM)
Lagerung
Store at -20°C
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.

Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit

g/mol

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