IF 13
PMID: 34569725 ↗Uridine
Catalog No.: GC13742Purity:>99.50%Synonyms: NSC 20256, 1-β-D-Ribofuranosyluracil, Uracil-1-β-D-ribofuranoside, β-Uridine
Uridin (β-Uridin) ist ein glykosyliertes Pyrimidin-Analogon, das Uracil enthÄlt, das Über eine β-N1-glykosidische Bindung an einen Ribosering (oder genauer gesagt Aribofuranose) gebunden ist.
Die Produkte sind ausschließlich für Forschungszwecke bestimmt. Nicht zur Anwendung am Menschen. Wir verkaufen nicht an Patienten.
Cas No.: 58-96-8
10mM (in 1mL DMSO)In Stock
$39.00
1
100mgIn Stock
$35.00
1
500mgIn Stock
$56.00
1
1gIn Stock
$79.00
1
5gIn Stock
$118.00
1
10gIn Stock
$155.00
1
25gIn Stock
$221.00
1
50gIn Stock
$276.00
1
100gIn Stock
$344.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $39.00 | In Stock | 1 | |
| 100mg | $35.00 | In Stock | 1 | |
| 500mg | $56.00 | In Stock | 1 | |
| 1g | $79.00 | In Stock | 1 | |
| 5g | $118.00 | In Stock | 1 | |
| 10g | $155.00 | In Stock | 1 | |
| 25g | $221.00 | In Stock | 1 | |
| 50g | $276.00 | In Stock | 1 | |
| 100g | $344.00 | In Stock | 1 |
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Zitate (Citations)
1 publicationsPublikationen von Forschern, die dieses Produkt verwendenBeschreibung (Description)
Uridine is a nucleoside that contains uracil and is linked to the ribose ring through a β-N1-glycosidic bond [1]. Uridine regulates enzymes and intermediates in the Uridine metabolism (such as UTP, DHODH, and UPase) and is associated with glucose homeostasis, lipid metabolism, and amino acid metabolism [2-3].
In vitro, Uridine (6-24mM; 7 days) dose-dependently inhibits the proliferation of leukemia cells HL-60 and induces HL-60 to differentiate into mature cells with monocyte and granulocyte characteristics [1]. Uridine (0.2-0.4mg/mL; 10h) reduces hepatocyte apoptosis caused by CCl4, significantly reduces the expression of Caspase-3 and Bax, and upregulates Bcl-2 expression [4].
In vivo, Uridine (10-20mg/kg; oral; 6 weeks) alleviates liver fibrosis in mice with CCl4-induced liver fibrosis model and inhibits the expression of pro-inflammatory cytokines and the activation of the NF-κB signaling pathway [4]. Uridine (30mg/kg; iv; single dose) significantly increases the UDP and UTP contents in the myocardium of rats with acute myocardial ischemia (AMI) and ischemia/reperfusion injury (I/R), reduces CrP and ATP levels, and significantly reduces the size of the ischemic area in the myocardium [5].
References:
[1] Sokoloski JA, et al., Effects of uridine on the growth and differentiation of HL-60 leukemia cells. Leuk Res. 1991;15(11):1051-8.
[2] Zhang Y, Guo S, Xie C, et al. Uridine metabolism and its role in glucose, lipid, and amino acid homeostasis[J]. BioMed research international, 2020, 2020(1): 7091718.
[3] Chenna Narendra S, Chalise J P, Magnusson M, et al. Local but not systemic administration of uridine prevents development of antigen-induced arthritis[J]. PloS one, 2015, 10(10): e0141863.
[4] Zheng W V, Li Y, Cheng X, et al. Uridine alleviates carbon tetrachloride‐induced liver fibrosis by regulating the activity of liver‐related cells[J]. Journal of Cellular and Molecular Medicine, 2022, 26(3): 840-854.
[5] Krylova IB, Selina EN, Bulion VV, et al. Uridine treatment prevents myocardial injury in rat models of acute ischemia and ischemia/reperfusion by activating the mitochondrial ATP-dependent potassium channel. Sci Rep. 2021;11(1):16999.
Protokoll (Protocol)
| Cell experiment [1]: | |
Cell lines | AML12 cells |
Preparation Method | To avoid the adverse effects of hepatocyte injury produced during perfusion, the mouse hepatocyte cell line (AML12, the mouse hepatocyte cell line) was also used to check the protective effects of Uridine on hepatocytes apoptosis caused by CCl4. Before 4h prior to treatment with 10mmol/L CCl4 for 10h, the hepatocytes were treated with Uridine (200µg/ml) or the vehicle (DMSO). Cell apoptosis rate was assessed using a PE Annexin V apoptosis detection kit according to the manufacturer's instructions. The cell samples were analysed with a FACSCalibur flow cytometer (Becton-Dickinson), and cells that are viable are Annexin V-PE and 7-AAD negative; cells that are in early apoptosis are Annexin V-PE positive and 7-AAD negative; and cells that are in late apoptosis or already dead are both Annexin V-PE and 7-AAD positive. |
Reaction Conditions | 200µg/ml; 10h |
Applications | Uridine reduced hepatocyte apoptosis induced by CCl4, significantly decreased the expression of Caspase-3 and Bax, and upregulated the expression of Bcl-2. |
| Animal experiment [1]: | |
Animal models | C57BL/6J mice |
Preparation Method | The establishment of the experimental mouse model was approved by the Animal Ethics Committee of the Intervention and Cell Therapy Center of Peking University Shenzhen Hospital. 30 healthy C57BL/6J mice were chosen (6–7 weeks old, weighing between 19 ± 2g) and randomly divided them into control group, model group and Uridine treatment group. Model group: CCl4 was mixed with olive oil into a 10% oily solvent, the mice from the model group were injected intraperitoneally with the mixed solution at 0.6μl/g twice per week for 8 weeks; Control group: the C57BL/6J mice were injected intraperitoneally with the same volume of olive oil at the same time point (as negative control); Uridine treatment group, the mice were given Uridine by drinking water (10–20mg/kg) for 6 weeks. Twenty‐four hours after the last injection, the eyeballs of experimental animals were removed and blood was collected. The mice were anaesthetized and perfused with saline. The liver tissues were collected for a series of biological analyses. |
Dosage form | 10-20mg/kg/day for 6 weeks; oral |
Applications | Uridine alleviated liver fibrosis in mice with CCl4-induced liver fibrosis model, and inhibited the expression of pro-inflammatory cytokines and the activation of the NF-κB signaling pathway. |
References: | |
Produktdokumente (Product Documents)
Purity:>99.50%Appearance:A solid
Chemische Eigenschaften (Chemical Properties)
CAS-Nr.
58-96-8
Synonyme
NSC 20256, 1-β-D-Ribofuranosyluracil, Uracil-1-β-D-ribofuranoside, β-Uridine
Chemischer Name
1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione
SMILES
C1=CN(C(=O)NC1=O)C2C(C(C(O2)CO)O)O
Summenformel
C9H12N2O6
Molekulargewicht
244.2
Löslichkeit
≥ 71.4mg/mL in DMSO
Lagerung
Store at 4°C
Allgemeine Hinweise
Wählen Sie anhand der Löslichkeitsdaten des Produkts ein geeignetes Lösungsmittel. Aliquotieren Sie die Stammlösung nach der Herstellung, um wiederholte Gefrier-Auftau-Zyklen zu vermeiden.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Verwenden Sie Stammlösungen bei Lagerung bei -80°C innerhalb von 6 Monaten bzw. bei -20°C innerhalb von 1 Monat.
Zur Verbesserung der Löslichkeit das Röhrchen auf 37°C erwärmen und für eine angemessene Dauer beschallen.
Versandbedingungen
Evaluierungsmuster werden mit Kühlakkus versandt. Andere verfügbare Größen werden bei Raumtemperatur oder auf Anfrage mit Kühlakkus versandt.
Rechner (Calculators)Molarität / Verdünnung / Molekulargewicht / Einheitenumrechnung / In-vivo-Formulierung / Löslichkeit
g/mol
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