Farnesol (Synonyms: Farnesol, FCI 119a, NSC 60597) |
Catalog No.GC32387
One-Click Copy Product Info
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Farnesol, a C15 terpene alcohol that is ubiquitous in nature, is a component of plant essential oils and is a quorum sensing molecule in Candida albicans.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 4602-84-0
Sample solution is provided at 25 µL, 10mM.
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Farnesol, a C15 terpene alcohol that is ubiquitous in nature, is a component of plant essential oils and is a quorum sensing molecule in Candida albicans [1]. Farnesol exerts the pro-inflammatory effect by increasing the levels of IL-6, TNF-α, and COX-2 in macrophage cells[2]. Farnesol has been widely used in various cell models and animal models to inhibit tumor growth[3].
In vitro, Farnesol treatment for 24 hours significantly inhibited the viability of A549 cells and Caco-2 cells, with IC50 values of 4.6mM and 3.9mM, respectively[4]. Treatment with 20μM Farnesol for 48 hours significantly inhibited the growth of MCF-7 cells, induced the expression of THRβ1 mRNA and protein, and prevented the binding of THRβ1 to DNA[5]. Treatment of H460 cells with 250μM Farnesol for 24 hours induced endoplasmic reticulum stress and unfolded protein response, promoted cell apoptosis, accompanied by the activation of MEK1/2[6].
In vivo, Farnesol treatment via oral administration at a dose of 100mg/kg/day for 5 weeks restored the cytokine secretion capacity of peritoneal macrophages in asthmatic mice, and reduced the levels of IL-6, TNF-α and inducible nitric oxide synthase (iNOS)[7]. Oral administration of 50mg/kg Farnesol daily for 5 weeks significantly reduced tumor growth in a prostate cancer mouse model[8].
References:
[1] Chandran S S, Kealey J T, Reeves C D. Microbial production of isoprenoids[J]. Process biochemistry, 2011, 46(9): 1703-1710.
[2] Ghosh S, Howe N, Volk K, et al. Candida albicans cell wall components and farnesol stimulate the expression of both inflammatory and regulatory cytokines in the murine RAW264. 7 macrophage cell line[J]. FEMS Immunology & Medical Microbiology, 2010, 60(1): 63-73.
[3] Jung Y Y, Hwang S T, Sethi G, et al. Potential anti-inflammatory and anti-cancer properties of farnesol[J]. Molecules, 2018, 23(11): 2827.
[4] Yilmaz Öztürk B, Feyzullazade N, Dağ İ, et al. The investigation of in vitro effects of farnesol at different cancer cell lines[J]. Microscopy Research and Technique, 2022, 85(8): 2760-2775.
[5] Duncan R E, Archer M C. Farnesol induces thyroid hormone receptor (THR) β1 but inhibits THR-mediated signaling in MCF-7 human breast cancer cells[J]. Biochemical and biophysical research communications, 2006, 343(1): 239-243.
[6] Joo J H, Liao G, Collins J B, et al. Farnesol-induced apoptosis in human lung carcinoma cells is coupled to the endoplasmic reticulum stress response[J]. Cancer research, 2007, 67(16): 7929-7936.
[7] Ku C M, Lin J Y. Farnesol, a sesquiterpene alcohol in herbal plants, exerts anti‐inflammatory and antiallergic effects on ovalbumin‐sensitized and‐challenged asthmatic mice[J]. Evidence‐Based Complementary and Alternative Medicine, 2015, 2015(1): 387357.
[8] Park J S, Kwon J K, Kim H R, et al. Farnesol induces apoptosis of DU145 prostate cancer cells through the PI3K/Akt and MAPK pathways[J]. International journal of molecular medicine, 2014, 33(5): 1169-1176.
| Cell experiment [1]: | |
Cell lines | Caco-2 cells |
Preparation Method | The growth and maintenance of Caco-2 cells were carried out in a modified Dubecco Eagle medium (DMEM) containing 10% FBS and 1% antibiotics, and the cells were cultured at 37°C, 5% CO2 and humidity conditions. Adjust the cell concentration to 5×104 cells/ml, and inoculate it in 96-well plates at a volume of 100μl/well. Treat the cells with different concentrations of Farnesol (0.01, 0.025, 0.05, 0.1, 0.5, 1, 2, 5, and 10mM) for 24 hours, and then analyze the cell viability. |
Reaction Conditions | 0.01, 0.025, 0.05, 0.1, 0.5, 1, 2, 5, and 10mM; 24h |
Applications | Farnesol treatment inhibited the cell viability of Caco-2 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male nude mice |
Preparation Method | Five-week-old male nude mice weighing 20-30 grams were raised in a standard sterile environment with a temperature of 23±5℃ and a relative humidity of 40±10%. A density of 5×106 cells/ml of DU145 cells were injected subcutaneously into the lateral abdomen of the nude mice. All mice received oral treatment starting from the day of tumor implantation and continued for 5 weeks. The control group mice were orally administered 0.2ml of corn oil daily for 5 weeks. The treatment group mice were orally administered 50mg/kg of Farnesol daily for 5 weeks, and the tumor growth was analyzed. |
Dosage form | 50mg/kg/day for 5 weeks; p.o. |
Applications | Farnesol treatment significantly decreased the tumor volume in the DU145 cell- xenograft mouse models. |
References: | |
| Cas No. | 4602-84-0 | SDF | |
| Synonyms | Farnesol, FCI 119a, NSC 60597 | ||
| Canonical SMILES | C/C(C)=C\CC/C(C)=C/CC/C(C)=C/CO | ||
| Formula | C15H26O | M.Wt | 222.37 |
| Solubility | DMF: 20 mg/ml,DMSO: 10 mg/ml,Ethanol: 30 mg/ml,Ethanol:PBS (pH 7.2) (1:3): 0.25 mg/ml | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.497 mL | 22.485 mL | 44.9701 mL |
| 5 mM | 899.4 μL | 4.497 mL | 8.994 mL |
| 10 mM | 449.7 μL | 2.2485 mL | 4.497 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A Liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)