2-Phospho-L-ascorbic acid trisodium salt

2-Phospho-L-ascorbic acid trisodium salt, a stable form of Ascorbic acid (Asc), is frequently employed as source of Asc in cell culture^[1]. 2-Phospho-L-ascorbic acid trisodium salt promotes osteoblast differentiation by enhancing collagen synthesis and alkaline phosphatase (ALP) activity^[2]. 2-Phospho-L-ascorbic acid trisodium salt has been widely used as a substrate for detecting target IgG and for determining hexavalent tungsten (W(VI)) in water^[3-4]. 2-Phospho-L-ascorbic acid trisodium salt has been widely used in the field of regenerative medicine to induce fibroblasts to produce extracellular matrix (ECM) for the preparation of tissue-engineered matrices^[5].

In vitro, 2-Phospho-L-ascorbic acid trisodium salt treatment (300μM) for 2 weeks promoted the proliferation of human corneal endothelial cells (HCECs) and reduced the level of 8-OHdG in mitochondrial DNA^[6]. Treatment with 100µM 2-Phospho-L-ascorbic acid trisodium salt for 3 weeks stimulated collagen production and promoted cell proliferation in skin fibroblasts^[7].

References:
[1] Liang L, He M, Zhang Y, et al. Unraveling the 2, 3-diketo-l-gulonic acid-dependent and-independent impacts of l-ascorbic acid on somatic cell reprogramming[J]. Cell & Bioscience, 2023, 13(1): 218.
[2] Takamizawa S, Maehata Y, Imai K, et al. Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells[J]. Cell biology international, 2004, 28(4): 255-265.
[3] Pham X H, Hahm E, Kim T H, et al. Enzyme-catalyzed Ag growth on Au nanoparticle-assembled structure for highly sensitive colorimetric immunoassay[J]. Scientific Reports, 2018, 8(1): 6290.
[4] Alvarado-Gámez A L, Alonso-Lomillo M A, Domínguez-Renedo O, et al. A chronoamperometric screen printed carbon biosensor based on alkaline phosphatase inhibition for W (VI) determination in water, using 2-phospho-l-ascorbic acid trisodium salt as a substrate[J]. Sensors, 2015, 15(2): 2232-2243.
[5] Brownell D, Carignan L, Alavi R, et al. Impact of the use of 2-phospho-L ascorbic acid in the production of engineered stromal tissue for regenerative medicine[J]. Cells, 2025, 14(14): 1123.
[6] Shima N, Kimoto M, Yamaguchi M, et al. Increased proliferation and replicative lifespan of isolated human corneal endothelial cells with L-ascorbic acid 2-phosphate[J]. Investigative ophthalmology & visual science, 2011, 52(12): 8711-8717.
[7] Hata R I, Senoo H. L‐ascorbic acid 2‐phosphate stimulates collagen accumulation, cell proliferation, and formation of a three‐dimensional tissuelike substance by skin fibroblasts[J]. Journal of cellular physiology, 1989, 138(1): 8-16.