GLPBio

CRF, bovine

Catalog No.: GC35748Purity:>98.00%
COASDSData Sheet
CRF, bovine is an important neuropeptide consisting of 41 amino acids, it is produced by the hypothalamus and is transported to the pituitary, where it triggers the release of adrenocorticotropic hormone (ACTH).

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CRF, bovine
Cas No.: 92307-52-3
500μgIn Stock
$200.00
1
1mgIn Stock
$322.00
1
5mgIn Stock
$1,101.00
1
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Description

CRF, bovine is an important neuropeptide consisting of 41 amino acids[1], it is produced by the hypothalamus and is transported to the pituitary, where it triggers the release of adrenocorticotropic hormone (ACTH)[2]. CRF, bovine plays a significant role in social behavior and is commonly utilized in research of various psychiatric disorders, such as depression, anxiety, and substance/alcohol abuse[3][4].

CRF, bovine (0.1µM; 3h) treatment can rapidly stimulated cyclic AMP production, increasing it 4- to 6-fold in 3min and reaching a peak of 10- to 15-fold at 30min in rat anterior pituitary cells[5]. In RAW 264.7 cells, treatment with CRF, bovine at 10nM for 4 hours enhanced the NF-κB-mediated luciferase expression[6].

CRF, bovine play important roles in sociability, intracerebroventricular administration of CRF, bovine (5µg/2µL; 30min) significantly decreased the number of entries and the time of interaction with the unknown male mice[7]. CRF (100ng; icv) consistently decreased immobility in the tail suspension test (TST) and the forced swim test (FST) in unshocked mice[8].

References:
[1]. Vale W, Spiess J, Rivier C, Rivier J. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 1981 Sep 18;213(4514):1394-7. doi: 10.1126/science.6267699. PMID: 6267699.
[2]. Hogg JE, Myers J, Hutson PH. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. doi: 10.1016/0014-2999(96)00470-0. PMID: 8894604.
[3]. Matsoukas MT, Panagiotopoulos V, Karageorgos V, et al. Structural and Functional Insights into CRF Peptides and Their Receptors. Biology (Basel). 2024 Feb 13;13(2):120. doi: 10.3390/biology13020120. PMID: 38392338; PMCID: PMC10886364.
[4]. Sanders J, Nemeroff C. The CRF System as a Therapeutic Target for Neuropsychiatric Disorders. Trends Pharmacol Sci. 2016 Dec;37(12):1045-1054. doi: 10.1016/j.tips.2016.09.004. Epub 2016 Oct 4. PMID: 27717506; PMCID: PMC5121012.
[5]. Aguilera G, Harwood JP, Wilson JX, et al. Mechanisms of action of corticotropin-releasing factor and other regulators of corticotropin release in rat pituitary cells. J Biol Chem. 1983 Jul 10;258(13):8039-45. PMID: 6305967.
[6]. Smith EM, Gregg M, Hashemi F, et al. Corticotropin Releasing Factor (CRF) activation of NF-kappaB-directed transcription in leukocytes. Cell Mol Neurobiol. 2006 Jul-Aug;26(4-6):1021-36. doi: 10.1007/s10571-006-9040-1. Epub 2006 Apr 22. PMID: 16633893; PMCID: PMC11520635.
[7]. Bagosi Z, Karasz G, Czébely-Lénárt A, et al. The effects of CRF and urocortins on the sociability of mice. Brain Res. 2017 May 15;1663:114-122. doi: 10.1016/j.brainres.2017.03.003. Epub 2017 Mar 14. PMID: 28315311.
[8]. Swiergiel AH, Leskov IL, Dunn AJ. Effects of chronic and acute stressors and CRF on depression-like behavior in mice. Behav Brain Res. 2008 Jan 10;186(1):32-40. doi: 10.1016/j.bbr.2007.07.018. Epub 2007 Jul 20. PMID: 17716752.

Protocol

Cell experiment [1]:

Cell lines

 Rat anterior pituitary cells

Preparation Method

 Rat anterior pituitary cells were treated with 0.1µM CRF, bovine for 3h. Then the cyclic AMP content was measured. For cyclic AMP measurement, cells were incubated for 30min with 1mM methylisobutylxanthine. Cell suspensions were boiled in equal volumes of PBS for 10min, then cooled and centrifuged at 1500g for 20min, the cyclic AMP content of the supernatant was measured by radioimmunoassay.

Reaction Conditions

 0.1µM; 3h

Applications

 CRF, bovine treatment can rapidly stimulated cyclic AMP production in rat anterior pituitary cells.
Animal experiment [2]:

Animal models

 Male CFLP mice

Preparation Method

 The mice were implanted with a stainless steel Luer cannula aimed at the right lateral cerebral ventricle, the mice were allowed for 5 days to recover after the surgery then treated with CRF, bovine. Thirty minutes after ICV injection of the CRF, bovine mice were investigated in a social interaction test arena.

Dosage form

 5µg/2µL; 30min; icv.

Applications

 CRF, bovine decreased the number of entries and the time of interaction with the unknown male.

References:
[1]. Aguilera G, Harwood JP, Wilson JX, et al. Mechanisms of action of corticotropin-releasing factor and other regulators of corticotropin release in rat pituitary cells. J Biol Chem. 1983 Jul 10;258(13):8039-45. PMID: 6305967.
[2]. Bagosi Z, Karasz G, Czébely-Lénárt A, et al. The effects of CRF and urocortins on the sociability of mice. Brain Res. 2017 May 15;1663:114-122. doi: 10.1016/j.brainres.2017.03.003. Epub 2017 Mar 14. PMID: 28315311.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
92307-52-3
Molecular Formula
C206H340N60O63S
Molecular Weight
4697.34
Solubility
Soluble in DMSO
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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