Soyasapogenol A |
Catalog No.GC37668
One-Click Copy Product Info
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Soyasapogenol A is a triterpenoid compound isolated from the roots of Abrus cantoniensis that exhibits anti-inflammatory, anti-cancer, hepatoprotective, and anti-HSV-1 activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 508-01-0
Sample solution is provided at 25 µL, 10mM.
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Soyasapogenol A is a triterpenoid compound isolated from the roots of Abrus cantoniensis that exhibits anti-inflammatory, anti-cancer, hepatoprotective, and anti-HSV-1 activities. Soyasapogenol A can be used in the research of tumors and immune inflammatory diseases[1][2][3].
In vitro, treatment with Soyasapogenol A at 10µM for 11 days caused a 68% reduction in MDA-MB-231 cell proliferation, whereas it stimulated a 2.4-fold increase in MCF-7 cell proliferation[4]. Treatment with Soyasapogenol A for 24h increased pS2 mRNA expression in MCF-7 cells by 3.4-fold at 0.1µM and by 5.5-fold at 1µM[4]. Treatment with Soyasapogenol A (0-0.5mg/mL) for 24-72h inhibited the proliferation of Hep-G2 cells, with an LC₅₀ of 0.052mg/mL, and induced cell cycle arrest and apoptosis[1].
In vivo, Soyasapogenol A was orally administered at a dose of 15mg/kg twice weekly for 6 weeks in SKOV-3 xenograft mice, demonstrating a potent and significant reduction in the growth of subcutaneous xenografts by more than 55% and lung metastases by more than 60%[5].
References:
[1] Zhang W, et al. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem. 2008 Apr 23;56(8):2603-8.
[2] Kuzuhara H, et al. Protective effects of soyasapogenol A on liver injury mediated by immune response in a concanavalin A-induced hepatitis model. Eur J Pharmacol. 2000 Mar 10;391(1-2):175-81.
[3] Guang C, et al. Biological functionality of soyasaponins and soyasapogenols. J Agric Food Chem. 2014 Aug 20;62(33):8247-55.
[4] Rowlands JC, Berhow MA, Badger TM. Estrogenic and antiproliferative properties of soy sapogenols in human breast cancer cells in vitro. Food Chem Toxicol. 2002;40(12):1767-1774.
[5]Omar A, Kalra RS, Putri J, Elwakeel A, Kaul SC, Wadhwa R. Soyasapogenol-A targets CARF and results in suppression of tumor growth and metastasis in p53 compromised cancer cells. Sci Rep. 2020;10(1):6323.
| Cell experiment [1]: | |
Cell lines | MCF-7 and MDA-MB-231 |
Preparation Method | Cell suspensions were diluted into a 24-well plate. The following day, Soyasapogenol A dissolved in DMSO were added. The final DMSO concentration did not exceed 0.3% (v/v). The cells were incubated in the Soyasapogenol A for a further 11 days in a 5% CO2 atmosphere and 100% humidity. The media with chemicals was changed after 4 days and then every 2 days thereafter. |
Reaction Conditions | 0, 0.1, 1, 10µM; 11d |
Applications | There were no significant changes in proliferation of MCF-7 or MDA-MB-231 cells cultured in 0.1 or 1.0µM SA for 11 days. However, at 10µM , Soyasapogenol A caused 68% reduction in MDA-MB-231 cell proliferation, whereas it stimulated a 2.4-fold increase in MCF-7 cell proliferation. |
| Animal experiment [2]: | |
Animal models | Athymic balb/c nude female mice transplanted with SKOV3 cells |
Preparation Method | Animals were pre-fed with either vehicle (0.1% carboxymethyl cellulose) or Soyasapogenol A supplemented vehicle (15mg/kg) in 250µL suspension twice a week before xenografting of SKOV-3 cells by subcutaneous and intravenous injections. Post-1 week of injections, mice were feed with Soyasapogenol A for next 4 weeks, before sacrifice. |
Dosage form | 15mg/kg; 2/w, 6w; p.o. |
Applications | Soyasapogenol A fed mice demonstrated a potent and significant reduction (>55%) in growth of subcutaneous xenografts and lung metastases (>60%). |
References: | |
| Cas No. | 508-01-0 | SDF | |
| Canonical SMILES | C[C@]12[C@]3(C([C@@]4([H])[C@](C)([C@@H]([C@H](O)C(C)(C)C4)O)CC3)=CC[C@]1([H])[C@@]5([C@@]([C@](C)([C@@H](O)CC5)CO)([H])CC2)C)C | ||
| Formula | C30H50O4 | M.Wt | 474.72 |
| Solubility | DMSO : 100 mg/mL (210.65 mM; Need ultrasonic) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1065 mL | 10.5325 mL | 21.065 mL |
| 5 mM | 421.3 μL | 2.1065 mL | 4.213 mL |
| 10 mM | 210.7 μL | 1.0533 mL | 2.1065 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)