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Coomassie Blue R-250 (Synonyms: C.I. 42660)

Catalog No.GC43309

Brilliant blue R250 (Brilliant Blue R), an anionic dye, is the most popular stain to detect proteins resolved in SDS-PAGE gels.

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Coomassie Blue R-250 Chemical Structure

Cas No.: 6104-59-2

Size Price Stock Qty
10g
$42.00
In stock
25g
$74.00
In stock
50g
$135.00
In stock
100g
$230.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Product Documents

Quality Control & SDS

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Background

Coomassie blue R-250 is a dye that is commonly used in laboratories to stain or quantify proteins. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins. Coomassie blue R-250 displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.

Chemical Properties

Cas No. 6104-59-2 SDF
Synonyms C.I. 42660
Canonical SMILES CC/[N+](CC1=CC(S([O-])(=O)=O)=CC=C1)=C(C=C/2)\C=CC2=C(C3=CC=C(N(CC)CC4=CC=CC(S([O-])(=O)=O)=C4)C=C3)\C(C=C5)=CC=C5NC6=CC=C(OCC)C=C6.[Na+]
Formula C45H44N3O7S2•Na M.Wt 826
Solubility DMF: 2 mg/ml,DMSO: 10 mg/ml,Ethanol: 0.1 mg/ml,PBS (pH 7.2): 1 mg/ml Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.2107 mL 6.0533 mL 12.1065 mL
5 mM 0.2421 mL 1.2107 mL 2.4213 mL
10 mM 0.1211 mL 0.6053 mL 1.2107 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

Review for Coomassie Blue R-250

Average Rating: 5 ★★★★★ (Based on Reviews and 10 reference(s) in Google Scholar.)

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