Taurohyocholic Acid (Synonyms: Tauro-γ-muricholic Acid) |
Catalog No.GC45574
One-Click Copy Product Info
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Taurohyocholic Acid is a taurine-conjugated bile acid derived from hyocholic acid, predominantly found in pig bile.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 32747-07-2
Sample solution is provided at 25 µL, 10mM.
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Taurohyocholic Acid is a taurine-conjugated bile acid derived from hyocholic acid, predominantly found in pig bile[1]. Taurohyocholic Acid plays a crucial role in glucose homeostasis[2] and metabolic regulation[3]. It is also used in research of oncology[4] and end-stage renal disease (ESRD)[5] as a biomarker for predicting treatment efficacy.
Taurohyocholic Acid stimulate GLP-1 production and secretion in intestinal enteroendocrine STC-1 and NCI-H716 cells by promoting proglucagon transcription. In STC-1 and NCI-H716 cells, treatment with 25µM Taurohyocholic Acid for 1 hour significantly increased GLP-1 secretion, and a 24-hour incubation further promoted proglucagon transcription and GLP-1 production[6].
References:
[1]. Fang W, Wen X, Meng Q, et al. Alteration in bile acids profile in Large White pigs during chronic heat exposure. J Therm Biol. 2019 Aug;84:375-383. doi: 10.1016/j.jtherbio.2019.07.027. Epub 2019 Jul 26. PMID: 31466777.
[2]. Cheng Z, Liu G, Zhang X, et al. Improvement of Glucose Metabolism Following Long-Term Taurocholic Acid Gavage in a Diabetic Rat Model. Med Sci Monit. 2018 Oct 9;24:7206-7212. doi: 10.12659/MSM.912429. PMID: 30298865; PMCID: PMC6192455.
[3]. Zheng X, Chen T, Zhao A, et al. Hyocholic acid species as novel biomarkers for metabolic disorders. Nat Commun. 2021 Mar 5;12(1):1487. doi: 10.1038/s41467-021-21744-w. PMID: 33674561; PMCID: PMC7935989.
[4]. Chen Y, Wang Y, Lei J, et al. Taurohyocholic acid acts as a potential predictor of the efficacy of tyrosine kinase inhibitors combined with programmed cell death-1 inhibitors in hepatocellular carcinoma. Front Pharmacol. 2024 Feb 23;15:1364924. doi: 10.3389/fphar.2024.1364924. PMID: 38464731; PMCID: PMC10920247.
[5]. Chen WY, Zhang JH, Chen LL, et al. Bioactive metabolites: a clue to the link between MASLD and CKD? Clin Mol Hepatol. 2024 Oct 21. doi: 10.3350/cmh.2024.0782. Epub ahead of print. PMID: 39428978.
[6]. Zheng X, Chen T, Jiang R, et al. Hyocholic acid species improve glucose homeostasis through a distinct TGR5 and FXR signaling mechanism. Cell Metab. 2021 Apr 6;33(4):791-803.e7. doi: 10.1016/j.cmet.2020.11.017. Epub 2020 Dec 17. PMID: 33338411.
| Cell experiment [1]: | |
Cell lines | STC-1 |
Preparation Method | The STC-1 cell were plated into 24 well-plates with an initial cell density of 2*105 cells/well, treated with 25µM Taurohyocholic Acid for 1h. The cell media was removed from the cells, centrifuged at 2,000g for 10min to remove cell debris and the supernatant was collected. An GLP-1 ELISA kit was used to measure GLP-1. |
Reaction Conditions | 25µM, 1h |
Applications | Taurohyocholic Acid can upregulated GLP-1 secretion in STC-1 cells. |
References: | |
| Cas No. | 32747-07-2 | SDF | |
| Synonyms | Tauro-γ-muricholic Acid | ||
| Chemical Name | 2-[[(3α,5β,6α,7α)-3,6,7-trihydroxy-24-oxocholan-24-yl]amino]-ethanesulfonic acid | ||
| Canonical SMILES | O[C@@H]1CC[C@@]2(C)[C@@]([C@@H](O)[C@@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC(NCCS(O)(=O)=O)=O)([H])C1 | ||
| Formula | C26H45NO7S | M.Wt | 515.7 |
| Solubility | 20mg/mL in ethanol, or DMSO, 30mg/mL in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9391 mL | 9.6956 mL | 19.3911 mL |
| 5 mM | 387.8 μL | 1.9391 mL | 3.8782 mL |
| 10 mM | 193.9 μL | 969.6 μL | 1.9391 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)