8-Epixanthatin |
Catalog No.GF17531
One-Click Copy Product Info
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8-Epixanthatin is a light-induced growth inhibitor that regulates the phototropic curvature in sunflower hypocotyls.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 30890-35-8
Sample solution is provided at 25 µL, 10mM.
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8-Epixanthatin is a light-induced growth inhibitor that regulates the phototropic curvature in sunflower hypocotyls [1]. 8-Epixanthatin inhibits auxin-induced growth of sunflower hypocotyl and oat coleoptile sections as well as the elongation of cress roots [2]. 8-Epixanthatin can be used as an internal reference to develop HPLC–UV or HPLC-MS/MS measurements for the examination of related compounds in plants[3].
In vitro, 8-Epixanthatin treatment for 12 hours significantly reduced the level of p-STAT3-Y705 in DU145 cells, with an IC50 value of 3.2μM[4].
In vivo, 8-Epixanthatin treatment via intraperitoneal injection at a dose of 50mg/kg, 5 times a week for 25 days, significantly inhibited tumor growth in the DU145 mouse xenograft model without affecting body weight[4].
References:
[1] Kühne M, Hamscher G, Körner U, et al. Formation of anhydrotetracycline during a high-temperature treatment of animal-derived feed contaminated with tetracycline[J]. Food chemistry, 2001, 75(4): 423-429.
[2] Yokotani-Tomita K, Kato J, Kosemura S, et al. Light-induced auxin-inhibiting substance from sunflower seedlings[J]. Phytochemistry, 1997, 46(3): 503-506.
[3] Spring O, Schmauder K, Lackus N D, et al. Spatial and developmental synthesis of endogenous sesquiterpene lactones supports function in growth regulation of sunflower[J]. Planta, 2020, 252(1): 2.
[4] Lee Y J, Choi J, Yoon Y J, et al. 8‐Epi‐xanthatin induces the apoptosis of DU145 prostate carcinoma cells through signal transducer and activator of transcription 3 inhibition and reactive oxygen species generation[J]. Phytotherapy Research, 2021, 35(3): 1508-1520.
| Cell experiment [1]: | |
Cell lines | DU145 cells |
Preparation Method | DU145 cells were grown in RPMI 1640 medium supplemented with 10% fetal bovine serum, 100U/ml penicillin, and 0.1mg/ml streptomycin in 5% CO2 at 37°C. 5×103 DU145 cells/ml were seeded into 96-well microplates for 24h. The wells were replenished with fresh complete medium containing different concentrations of 8-Epixanthatin (0, 2, 5, 10, and 20µM) or 0.1% DMSO for 24h. Then, the cell viability was detected. |
Reaction Conditions | 0, 2, 5, 10, and 20µM; 24h |
Applications | 8-Epixanthatin treatment significantly reduced the cell viability of DU145 cells in a dose-dependent manner. |
| Animal experiment [1]: | |
Animal models | Female BALB/c mice |
Preparation Method | Female BALB/c mice (6 weeks old) were kept in temperature-controlled (21°C-25°C) and humidity-maintained (40%-70%) rooms with a 12-h light/dark cycle. Water and food were provided freely. DU145 cells (9×106 cells/mouse) were subcutaneously injected into the right flank of mice. Tumor growth was measured with calipers using the formula volume=1/2 (length×width×height). Vehicle and 8-Epixanthatin (50mg/kg) were intraperitoneally injected 5 days per week for 25 days starting on day 1 (the day after cell injection; six mice per group). On day 25, the mice were sacrificed, and the tumors were removed and weighed. |
Dosage form | 50mg/kg; 5 times a week for 25 days; i.p. |
Applications | 8-Epixanthatin treatment significantly inhibited tumor growth in the DU145 mouse xenograft model. |
References: | |
| Cas No. | 30890-35-8 | SDF | |
| Formula | C15H18O3 | M.Wt | 246.302 |
| Solubility | DMSO : 50 mg/mL (203.00 mM; Need ultrasonic) | Storage | 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0601 mL | 20.3003 mL | 40.6006 mL |
| 5 mM | 812 μL | 4.0601 mL | 8.1201 mL |
| 10 mM | 406 μL | 2.03 mL | 4.0601 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)