HBC620 |
| Catalog No.GC60186 |
HBC620 is an analog of HBC, which is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2530162-07-1
Sample solution is provided at 25 µL, 10mM.
HBC620 is an analog of HBC, which is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, but strongly fluoresces upon constraining intramolecular motion. HBC-Pepper complex can be used to visualize RNA dynamics in live cells[1].
HBC's fluorescence can be enhanced more than 3000-fold in vitro by Pepper. Mammalian cells expressing Pepper exhibited robust greenyellowish fluorescence on incubation with 1μM HBC[1].
References:
[1].Chen X, Zhang D, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293.
| Cell experiment [1]: | |
Cell lines | 293T/17 cells |
Preparation Method | 293T/17 cells were transfected with the corresponding plasmids. After 30h, the cells were digested and resuspended in the 4% FBS/1× PBS solution containing 0.2μM HBC and 5mM MgSO4. The fluorescence was analyzed using a CytoFLEX-S flow cytometer. |
Reaction Conditions | 0.2μM HBC |
Applications | When cells co-expressing Pepper-tagged centromere-targeting sgRNA and dCas9-GFP were incubated with the HBC620 fluorophore, numerous (~40) nuclear foci of Pepper620 fluorescence nearly perfectly co-localized with GFP fluorescence. |
References: | |
| Cas No. | 2530162-07-1 | SDF | |
| Canonical SMILES | OCCN(C)C1=CC(S2)=C(S1)C=C2/C=C(C#N)/C3=CC=C(C#N)C=C3 | ||
| Formula | C19H15N3OS2 | M.Wt | 365.47 |
| Solubility | DMSO:16.67 mg/mL (45.61 mM) | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.7362 mL | 13.681 mL | 27.362 mL |
| 5 mM | 0.5472 mL | 2.7362 mL | 5.4724 mL |
| 10 mM | 0.2736 mL | 1.3681 mL | 2.7362 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)
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