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HBC620

Catalog No.GC60186

HBC620 Chemical Structure

HBC620 is an analog of HBC, which is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor.

Size Price Stock Qty
5mg
$278.00
In stock
10mg
$445.00
In stock
50mg
$1,298.00
In stock
100mg
$2,039.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

293T/17 cells

Preparation Method

293T/17 cells were transfected with the corresponding plasmids. After 30h, the cells were digested and resuspended in the 4% FBS/1× PBS solution containing 0.2μM HBC and 5mM MgSO4. The fluorescence was analyzed using a CytoFLEX-S flow cytometer.

Reaction Conditions

0.2μM HBC

Applications

When cells co-expressing Pepper-tagged centromere-targeting sgRNA and dCas9-GFP were incubated with the HBC620 fluorophore, numerous (~40) nuclear foci of Pepper620 fluorescence nearly perfectly co-localized with GFP fluorescence.

References:

[1]. Chen X, Zhang D, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293..

Background

HBC620 is an analog of HBC, which is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, but strongly fluoresces upon constraining intramolecular motion. HBC-Pepper complex can be used to visualize RNA dynamics in live cells[1].

HBC's fluorescence can be enhanced more than 3000-fold in vitro by Pepper. Mammalian cells expressing Pepper exhibited robust greenyellowish fluorescence on incubation with 1μM HBC[1].

References:
[1].Chen X, Zhang D, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293.

Chemical Properties

Cas No. 2530162-07-1 SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES OCCN(C)C1=CC(S2)=C(S1)C=C2/C=C(C#N)/C3=CC=C(C#N)C=C3
Formula C19H15N3OS2 M.Wt 365.47
Solubility DMSO:16.67 mg/mL (45.61 mM) Storage Store at -20°C,unstable in solution, ready to use.
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Average Rating: 5 ★★★★★ (Based on Reviews and 9 reference(s) in Google Scholar.)

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