HBC620
Products are for research use only. Not for human use. We do not sell to patients.
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 5mg | $158.00 | In Stock | 1 | |
| 10mg | $253.00 | In Stock | 1 |
Citations
3 publicationsPublications from researchers using this productDescription
HBC620 is an HBC-like fluorophore, a GFP fluorophore-like synthetic dye, with a maximum excitation wavelength of 577nm and a maximum emission wavelength of 620nm [1]. HBC620 can be used as an RNA tag, which is bright, stable, and colorful, and capable of imaging a variety of RNAs in living cells[2]. The unique fluorescence imaging channel provided by HBC620 has been widely used in the engineering of luminometric sensor platforms for multiple miRNA imaging[3]. HBC620 can be used for in situ localization of tumor-specific protease and animal optical in vivo imaging analysis experiments[4].
References:
[1] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] Tang H, Peng J, Peng S, et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. Chemical Science, 2022, 13(47): 14032-14040.
[3] Yin P, Ge M, Xie S, et al. A universal orthogonal imaging platform for living-cell RNA detection using fluorogenic RNA aptamers[J]. Chemical Science, 2023, 14(48): 14131-14139.
[4] Xie X, Sun Y, Peng J, et al. Collagen Anchoring Protein-Nucleic Acid Chimeric Probe for In Situ In Vivo Mapping of a Tumor-Specific Protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
Protocol
HBC620-based In Vivo Mapping of a Tumor-Specific Protease
This protocol comes from the literature, please make modifications according to your specific needs.
1. Establish the xenograft tumor mouse model
(1) Male BALB/c nude mice (6 weeks of age; weighing 18-20g); 1×106 B16 cells were injected subcutaneously into the right armpit of each mouse, and the tumor volume reached 80-100mm3 (about 7-8 days).
(2) Chimeric probes were administered to the tail vein of mice: CBD-miRFP-DCV chimeric probes were dissolved in 200μl PBS and slowly pushed at 29G of the tail vein for 30s (1nM/20g).
2. Preparation and application of HBC620
(1) 5μM HBC620 working solution was prepared from 0.5mM HBC620 stock solution (dissolved in PBS, filtered at 0.22μm), use immediately.
(2) After anesthesia, the mice were depilated, and 200μl HBC620 working solution (final dose 1nM/20g, 1:1 molar ratio to probe) was injected into the tail vein.
3. In vivo imaging analysis
(1) Real-time acquisition: 0min, 5min, 15min, 30min, 1h, 2h, 4h, 8h, 24h; Parameters: excitation wavelength at 570nm, emission wavelength at 650-680nm, exposure at 500ms, f/stop 2, field of view 12cm×12cm.
(2) Quantification: tumor ROI and ipsilateral back normal skin ROI were selected to calculate T/N ratio (tumor-to-normal ratio).
4. Caveats.
(1) Dark operation: HBC620 working solution has a half-life of 4h at 4°C, and is prepared and used on demand.
(2) The laser power was ≤0.5W/cm2 to prevent tissue thermal damage.
(3) If imaging needs to be repeated longitudinally, the interval is ≥48h to ensure that the first dye has been removed (24-hour urinary excretion > 85%).
References:
[1] Xie X, Sun Y, Peng J, et al. Collagen Anchoring Protein-Nucleic Acid Chimeric Probe for In Situ In Vivo Mapping of a Tumor-Specific Protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
Product Documents
Chemical Properties
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
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