HBC620 |
Catalog No.GC60186
One-Click Copy Product Info
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HBC620 is an HBC-like fluorophore, a GFP fluorophore-like synthetic dye, with a maximum excitation wavelength of 577nm and a maximum emission wavelength of 620nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2530162-07-1
Sample solution is provided at 25 µL, 10mM.
HBC620 is an HBC-like fluorophore, a GFP fluorophore-like synthetic dye, with a maximum excitation wavelength of 577nm and a maximum emission wavelength of 620nm [1]. HBC620 can be used as an RNA tag, which is bright, stable, and colorful, and capable of imaging a variety of RNAs in living cells[2]. The unique fluorescence imaging channel provided by HBC620 has been widely used in the engineering of luminometric sensor platforms for multiple miRNA imaging[3]. HBC620 can be used for in situ localization of tumor-specific protease and animal optical in vivo imaging analysis experiments[4].
References:
[1] Chen X, Zhang D, Su N, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs[J]. Nature Biotechnology, 2019, 37(11): 1287-1293.
[2] Tang H, Peng J, Peng S, et al. Live-cell RNA imaging using the CRISPR-dCas13 system with modified sgRNAs appended with fluorescent RNA aptamers[J]. Chemical Science, 2022, 13(47): 14032-14040.
[3] Yin P, Ge M, Xie S, et al. A universal orthogonal imaging platform for living-cell RNA detection using fluorogenic RNA aptamers[J]. Chemical Science, 2023, 14(48): 14131-14139.
[4] Xie X, Sun Y, Peng J, et al. Collagen Anchoring Protein-Nucleic Acid Chimeric Probe for In Situ In Vivo Mapping of a Tumor-Specific Protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
HBC620-based In Vivo Mapping of a Tumor-Specific Protease
This protocol comes from the literature, please make modifications according to your specific needs.
1. Establish the xenograft tumor mouse model
(1) Male BALB/c nude mice (6 weeks of age; weighing 18-20g); 1×106 B16 cells were injected subcutaneously into the right armpit of each mouse, and the tumor volume reached 80-100mm3 (about 7-8 days).
(2) Chimeric probes were administered to the tail vein of mice: CBD-miRFP-DCV chimeric probes were dissolved in 200μl PBS and slowly pushed at 29G of the tail vein for 30s (1nM/20g).
2. Preparation and application of HBC620
(1) 5μM HBC620 working solution was prepared from 0.5mM HBC620 stock solution (dissolved in PBS, filtered at 0.22μm), use immediately.
(2) After anesthesia, the mice were depilated, and 200μl HBC620 working solution (final dose 1nM/20g, 1:1 molar ratio to probe) was injected into the tail vein.
3. In vivo imaging analysis
(1) Real-time acquisition: 0min, 5min, 15min, 30min, 1h, 2h, 4h, 8h, 24h; Parameters: excitation wavelength at 570nm, emission wavelength at 650-680nm, exposure at 500ms, f/stop 2, field of view 12cm×12cm.
(2) Quantification: tumor ROI and ipsilateral back normal skin ROI were selected to calculate T/N ratio (tumor-to-normal ratio).
4. Caveats.
(1) Dark operation: HBC620 working solution has a half-life of 4h at 4°C, and is prepared and used on demand.
(2) The laser power was ≤0.5W/cm2 to prevent tissue thermal damage.
(3) If imaging needs to be repeated longitudinally, the interval is ≥48h to ensure that the first dye has been removed (24-hour urinary excretion > 85%).
References:
[1] Xie X, Sun Y, Peng J, et al. Collagen Anchoring Protein-Nucleic Acid Chimeric Probe for In Situ In Vivo Mapping of a Tumor-Specific Protease[J]. Analytical Chemistry, 2023, 95(50): 18487-18496.
| Cas No. | 2530162-07-1 | SDF | |
| Canonical SMILES | OCCN(C)C1=CC(S2)=C(S1)C=C2/C=C(C#N)/C3=CC=C(C#N)C=C3 | ||
| Formula | C19H15N3OS2 | M.Wt | 365.47 |
| Solubility | DMSO:16.67 mg/mL (45.61 mM) | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.7362 mL | 13.681 mL | 27.362 mL |
| 5 mM | 547.2 μL | 2.7362 mL | 5.4724 mL |
| 10 mM | 273.6 μL | 1.3681 mL | 2.7362 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 9 reference(s) in Google Scholar.)